血小板膜糖蛋白Ibα基因HPA-2、Kozak序列多态性与脑梗死的相关性研究

目的探讨血小板膜糖蛋白(platelet membrane glycoprotein，GP) Ibα基因HPA-2、Kozak序列多态性与脑梗死的相关性。方法选取316例经CT/MRI证实的脑梗死患者做病例组，209例与病例组年龄、性别相匹配的无脑血管病人群做对照组。采用聚合酶链反应-限制性片断长度多态性(polymerase chain reactionrestriction fragment length polymorphism，PCR-RFLP）技术检测人类血小板抗原-2（human platelet antigen-2，HPA-2）及Kozak序列多态性在两组中的分布频率。结果病例组GPIbα基因HPA-2等位基因频率、基因型与对照组相比差异无统计学意义（P＞0.05）。病例组Kozak序列C等位基因频率为25.95%，对照组C等位基因频率为18.18%，两组间差异具有统计学意义（χ2=8.1323, P＜0.01）。结论GPIbα基因HPA-2序列多态性与脑梗死无相关性；Kozak序列多态性可能是脑梗死的遗传易感因素。

Relationship between the HPA-2 and Kozak sequence polymorphism of platelet membrane glycoprotein and cerebral infarction

To explore the relationship between polymorphism of platelet membrane glycoprotein I bα HPA-2 and Kozak sequence and cerebral infarction in the Chinese. MethodsThree hundred and sixteen patients with cerebral infarction confirmed by CT/MRI and two hundred and nine controls were genotyped for the HPA-2 and Kozak polymorphism by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis. ResultsThere were no-significant differences in the HPA-2 genotype frequencies and allele frequencies between cerebra vascular thrombosis patients and controls． The Kozak genotypes with the C allele were 25.95% in the cerebral infarction group compared with those of the control group（18.18%）. Statistical signs showed significant differences (χ2=8.6090, P＜0.01). ConclusionsPlatelet glycoprotein I bα polymorphisms are not associated with cerebral vascular thrombosis in our cohort. The Kozak genotype may be the genetic susceptibility of cerebral vascular thrombosis.