青蒿琥酯诱导人脐静脉内皮细胞凋亡

目的　探讨青蒿琥酯对人脐静脉内皮细胞(HUVEC)的诱导凋亡作用 ,以及这种诱导作用与内皮细胞增殖及细胞内游离Ca2 + 的关系。方法　采用MTT比色法测定青蒿琥酯对HUVEC增殖的影响 ,Fura 2 /AM荧光标记法测定HUVEC胞内游离Ca2 + 浓度 ([Ca2 + ]i) ,应用吖啶橙 /溴化乙啶荧光双染色、DNA琼脂糖凝胶电泳、流式细胞术检测细胞凋亡。结果　青蒿琥酯可抑制HUVEC增殖 ,明显诱导HUVEC凋亡 ,并呈浓度依赖性和时间依赖性效应关系。在发生凋亡的同时伴有 [Ca2 + ]i 显著升高。结论　青蒿琥酯可诱导HUVEC凋亡 ,其机制可能与[Ca2 + ]i 升高有关

Apoptosis of human umbilical vein endothelial cells induced by artesunate

AIM To investigate if the antitumor effect of artesunate is related to the induction of apoptosis in endothelial cells. METHODS The antiproliferation activities of artesunate were evaluated on human umbilical vein endothelial cells(HUVEC) by means of MTT vital staining. The induction of apoptosis was detected by acridine orange/ethidium bromide dual staining as well as DNA electrophoresis. The ratios of apoptosis in HUVEC and distribution of cell cycle phases were assessed by flow cytometry. The intracellular free calcium concentration ([Ca 2 ] i) of HUVEC was assayed with fluorescence spectroscopic analysis. RESULTS Artesunate suppressed HUVEC proliferation, induced apoptosis of HUVEC, changed the distribution of cell cycle phases, and increased [Ca 2 ] i remarkably. CONCLUSION Artesunate induces apotosis of HUVEC, which may be associated with the increase of [Ca 2 ] i.