| 树鼩腺病毒（TAV）PCR方法的建立及初步应用 |
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| 引用本文： | 王淑菁,付瑞,李晓波,王吉,卫礼,巩薇,贺争鸣,岳秉飞.树鼩腺病毒（TAV）PCR方法的建立及初步应用[J].中国比较医学杂志,2014,24(12):42-46. |
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| 作者姓名： | 王淑菁 付瑞 李晓波 王吉 卫礼 巩薇 贺争鸣 岳秉飞 |
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| 作者单位： | 中国食品药品检定研究院实验动物资源研究所;国家实验动物质量检测中心 |
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| 基金项目： | 实验动物新品种的种群建立与质量标准化研究(2011BAI15B01);实验动物质量检测关键技术研究(2013BAK11B01)。 |
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| 摘 要： | 目的建立树鼩腺病毒(TAV)PCR检测方法,并进行初步应用。方法根据NCBI公布的TAV基因组序列,选择19418-19917区域人工合成一段DNA序列并转入质粒中,作为阳性标准质粒。设计1对特异性引物建立TAV PCR检测方法,考察其特异性和灵敏度。应用该PCR方法对60份树鼩血DNA及56份树鼩粪便DNA进行检测。结果所建立的PCR检测方法经特异性和灵敏度测定,最低可检测13.5 x10-7μg/m L,与其他腺病毒无交叉,特异性好,灵敏度较高。60份树鼩血DNA检测均为阴性,56份树鼩粪便DNA检测有24份阳性,通过测序证实树鼩粪便中TAV感染率为42.9%,与扩增结果一致。结论建立的树鼩腺病毒PCR检测方法特异性强,灵敏度高,可用于树鼩腺病毒的常规检测中。
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| 关 键 词： | 树鼩 腺病毒 PCR 检测 |
| 收稿时间： | 2014/9/15 0:00:00 |
| 修稿时间： | 2014/9/27 0:00:00 |
Establishment and application of a PCR method for detection of the Tupaia (tree shrew) adenovirus (TAV) |
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| wangshujing,furui,lixiaobo,wangji,weili,gongwei,hezhengming and yuebingfei.Establishment and application of a PCR method for detection of the Tupaia (tree shrew) adenovirus (TAV)[J].Chinese Journal of Comparative Medicine,2014,24(12):42-46. |
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| Authors: | wangshujing furui lixiaobo wangji weili gongwei hezhengming and yuebingfei |
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| Institution: | National Institute for Food and Drug Control, Institute for Laboratory Animal Resources; National Center for Monitoring of Laboratory Animal Health, Beijing 100050, China;National Institute for Food and Drug Control, Institute for Laboratory Animal Resources; National Center for Monitoring of Laboratory Animal Health, Beijing 100050, China;National Institute for Food and Drug Control, Institute for Laboratory Animal Resources; National Center for Monitoring of Laboratory Animal Health, Beijing 100050, China;National Institute for Food and Drug Control, Institute for Laboratory Animal Resources; National Center for Monitoring of Laboratory Animal Health, Beijing 100050, China;National Institute for Food and Drug Control, Institute for Laboratory Animal Resources; National Center for Monitoring of Laboratory Animal Health, Beijing 100050, China;National Institute for Food and Drug Control, Institute for Laboratory Animal Resources; National Center for Monitoring of Laboratory Animal Health, Beijing 100050, China;National Institute for Food and Drug Control, Institute for Laboratory Animal Resources; National Center for Monitoring of Laboratory Animal Health, Beijing 100050, China;National Institute for Food and Drug Control, Institute for Laboratory Animal Resources; National Center for Monitoring of Laboratory Animal Health, Beijing 100050, China |
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| Abstract: | |
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| Keywords: | Tupaia (tree shrew) Adenovirus PCR Detection |
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