不同产地槐米中芦丁的含量测定

目的：通过测定不同产地槐米中主要活性成分芦丁的含量,评价槐米质量优劣。方法：采用高效液相色谱法,Welch XB-C18柱（5μm,4.6mm×250mm）,流动相为甲醇和1%冰醋酸水溶液（39：61）,检测波长257nm,柱温30℃,流速1.2mL∕min。结果：该方法标准曲线良好,线性方程为Y=12243X-80.436（r=0.9999）,平均加样回收率为99.15%,RSD为1.6%。不同产地槐米中芦丁含量在13.88%～35.74%之间。结论：不同产地槐米中芦丁含量差异显著,其中以广西桂林地区所产槐米质量较佳。

Determination of Rutin in Flos Sophorae From Different Growing Areas

Objective：To evaluate the quality of Flos Sophorae through determining the content of rutin which is the main active ingredient of Flos Sophorae from different growing areas.Methods：HPLC analysis was made on a Welch XB-C18（5 μm,4.6 mm ×250 mm） with isocratic elution 1% acetic acid-methanol（61：39） as the mobile phase was at a flow-rate of 1.2 mL∕min.The detection wavelength was set at 257 nm,and the column temperature was 30 ℃.Results：The linear equation was Y = 12243X-80.436（r = 0.9999）,and the average recovery was 99.15%,RSD = 1.6%.The content of Rutin in Flos Sophorae from different growing areas were 13.88% to 35.74%.Conclusion：There is variation of the rutin content in Flos sophorae from different growing areas,and the quality of samples from Guangxi Guilin were higher than those from other places.