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| 1-溴丙烷引发雄性大鼠性腺基因表达谱的变化 | |
| 引用本文： | 李卫华,市原学,王海兰,Kitagawa Emiko,丁训诚,周志俊.1-溴丙烷引发雄性大鼠性腺基因表达谱的变化[J].卫生研究,2010,39(2). |
| 作者姓名： | 李卫华 市原学 王海兰 Kitagawa Emiko 丁训诚 周志俊 |
| 作者单位： | 1. 上海市计划生育科学研究所 2. 日本名古屋大学医学部,日本,466-8550 3. 广东省职业病防治院,广州,510300 4. 日本产业技术综合研究所,日本,305-8568 5. 上海市计划生育科学研究所,上海,200032 6. 复旦大学公共卫生学院教育部公共卫生安全重点实验室,上海,200032 |
| 摘 要： | 目的研究1-溴丙烷(1-BP)诱发大鼠性腺基因表达谱的变化,探索1-BP雄性生殖毒性相关的mRNA改变。方法雄性F344/NSIc大鼠12只,随机分为2组,分别吸入新鲜空气或5030mg/m31-BP8h。染毒后16h处死大鼠取出睾丸,运用大鼠性腺cDNA微阵列和real-timePCR方法测定1-BP染毒后性腺相关基因表达谱的变化。结果在大鼠性腺芯片5087个cDNA微点阵中,有62个基因被1-BP显著下调,3个基因显著上调。其中包括性激素合成相关基因细胞色素P450芳香化酶(CYP19a),谷胱苷肽S-转移酶(GSTT1),肌酸激酶(Ckb),髓鞘和淋巴细胞蛋白(Mal)和S100的钙结合蛋白(S100a4)。归类分析结果显示绝大多数变化的基因与蛋白质/脂类代谢相关,其次与应激防御反应相关。实时定量PCR证实了1-BP可引起CYP19a、S100a4、GSTT1和Mal的下调。结论急性高剂量染毒1-BP可引起睾丸组织CYP19a、S100a4、GSTT1、Mal等基因的下调,提示其可能通过内分泌干扰和氧化应激效应而导致雄性生殖毒性。 |
| 关 键 词： | 1-溴丙烷 生殖毒性 性腺 基因芯片 实时定量PCR 环境毒理 |
Change of gonad gene expression profile in male F344 rats after exposure to 1-bromopropane |
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| LI Weihua,ICHIHARA Gaku,WANG Hailan,Kitagawa Emiko,Iwahashi Hitoshi,DING Xuncheng,ZHOU Zhijun.Change of gonad gene expression profile in male F344 rats after exposure to 1-bromopropane[J].Journal of Hygiene Research,2010,39(2). | |
| Authors: | LI Weihua ICHIHARA Gaku WANG Hailan Kitagawa Emiko Iwahashi Hitoshi DING Xuncheng ZHOU Zhijun |
| Institution: | LI Weihua,ICHIHARA Gaku,WANG Hailan,Kitakawa Emiko,Iwahashi Hitoshi,DING Xuncheng,ZHOU Zhijun School of Public Health,MO Key Lab. for Public Health Safety,Fudan University,Shanghai 200032,China |
| Abstract: | Objective To study the 1-bromopropane (1-BP)-induced altered gene expression profiles in rat gonad,and explore its male reproductive toxicity-related mRNA changes. Methods Twelve F344 /NSIc male rats were randomly divided into two groups of 6 each. Rats were exposed to either fresh air or 5030 mg /m3 1-BP through inhalation for 8 h. Rats were sacrificed and testes were removed at 16 h after exposure. Global changes in gene expression were determined by microarray analysis using rat genital chip followed by ... |
| Keywords: | 1-bromopropane reproductive toxicity testis cDNA microarray real-time PCR |
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