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| 我国SEO型汉坦病毒L基因核苷酸序列测定研究 | |
| 引用本文： | 郭彦敏,安洪明,马士恒,王华,杭长寿. 我国SEO型汉坦病毒L基因核苷酸序列测定研究[J]. 中国公共卫生, 2001, 17(8): 684-686 |
| 作者姓名： | 郭彦敏 安洪明 马士恒 王华 杭长寿 |
| 作者单位： | 1. 河北省保定市传染病医院, 2. 保定市急救中心 3. 河北省职工医学院 4. 中国预防医学科学院病毒学研究所 |
| 摘 要： | 目的：为各基因型或亚型病毒生物学表型研究打基础，对疫苗的选择使用提供科学依据。方法：将获得SEO型病毒分离、MCABS分型RT－PCR扩增及核苷酸序列测定。结果：首次测出我国SEO型病毒部分L基因核苷酸序列，并与76－118、C4、L99、Seoul株的核苷酸及氨基酸同源性进行比较，分别为70．8％、72％、95．3％、95．9％及75．6％、751％、97．7％、97．3％。结论：病毒基因组间的重排不是造成发病高峰的原因。 |
| 关 键 词： | 肾综合征出血热 汉坦病毒 核苷酸序列 测定 中国 |
| 文章编号： | 1001-0580(2001)08-0684-03 |
| 修稿时间： | 2000-06-13 |
Study on L Gene Necleotide Sequence of Hautaan SEO Type Virus |
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| GUO Yan min,et al.. Study on L Gene Necleotide Sequence of Hautaan SEO Type Virus[J]. Chinese Journal of Public Health, 2001, 17(8): 684-686 | |
| Authors: | GUO Yan min et al. |
| Affiliation: | GUO Yan min,et al.Baoding Infectious Diseases Hospital |
| Abstract: | Objective To lay basis for studing and to provide scientific basis for selecting vaccine SEO viral genes.Methods Viral isolation MCABS typing and RT-PCR were used to determinate a SEO strain.Nucleotide sequence of its L gene was detected.Results The nucleotide sequence of partial L region of SEO virus was acquired for the first time in China.Compared with 76-118 C4 L99 and Seoul strain,the homologies of nucleotide sequence were 70 8%?72%?95 3%?95 9%,respectively,amino acid sequences were 75 6%?75 1%?97 7%?97 3% repectively.Conclusion The high epidemics of HFRS wasn't because of recombinant of viral genes. |
| Keywords: | hemorrhagic fever with renal syndrome(HFRS) hantaan virus necleic and sequence |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |