三氯化铝对小鼠学习记忆和大鼠海马脑片长时程增强的影响

采用 icv1 % Al Cl3(每侧 2 μL,隔天注射 )的方法做成小鼠学习记忆障碍模型 ,观察了 Al Cl3对学习记忆行为的影响 ,并用微电极方法观察了不同浓度 Al Cl3(0 .0 1 - 1 0 0 μmol·L-1)对大鼠海马脑片长时程增强 (LTP)的影响 .结果显示 ,在跳台与避暗实验中 ,模型组小鼠错误次数明显增加 ,潜伏期明显缩短 ,但自发活动计数均无显著变化 .脑片实验中 ,Al Cl31 .0和 1 0 0 μmol·L-1浓度组明显抑制海马脑片产生 LTP.结果提示隔天大剂量 Al Cl3icv可降低学习记忆能力

Effect of aluminum trichloride on learning and memory in mice and the long- term potentiation of hippocampal slices of rats

The effect of 1% AlCl₃(2 μL) injected in each lateral ventricle of mice (one lateral on d 1 and the other lateral on d 3) on learning and memory was studied. The results showed that in the step-down and step- through test, the number of error was obviously increased and the latency was obviously shortened without obvious change in locomotor activity. In the experiment on hippocampal slices of rats in vitro by microelectrode method, AlCl₃ 1.0 and 100 μmol·L^-1 inhibited the generation of long- term potentiation induced by tetanic stimulation (100 Hz, 1 ms, for 1 s). The results suggest that AlCl₃ cause an evident learning and memory disturbance by icv every other day in large dose.