可见分光光度法测定玉簪花中总皂苷的含量

目的 建立玉簪花总皂苷的含量测定方法.方法 采用可见分光光度法,以香草醛-冰醋酸-浓硫酸为显色剂,支脱皂苷元为对照品,在450 nm波长下测定吸光度,标准曲线法计算供试品中总皂苷的含量.结果 支脱皂苷元的质量浓度在14～70 mg/L范围内与吸光度呈良好的线性关系(r=0.999 2),平均加样回收率为96.8%,RSD=2.97%(n=6).结论 该法操作简便、准确、灵敏、重复性好,可用于玉簪花药材中总皂苷的含量测定.

Quantitative determination of the total saponins from Flos Hostae Plantagineae by ultraviolet-visible spectrophotometry

Objective To set up a ultraviolet-visible spectrophotometry for quantitative determination of the total saponins from Flos Hostae Plantagineae. Methods The ultraviolet-visible spectrophotometry was used to detect the absorbance with the wavelength of 450 nm taking vanillin-glacial acetic acid-concentrated sulphuric acid as the color-developing agent and gitogenin as the control sample. The content of the total saponins in the samples was calculated by using standard curve. Results Gitogenin showed a good linear relationship at range of 14～70 μg/ml (r=0.999 2) with the average recovery of 96.8% (RSD=2.97%, n=6). Conclusion The method is easy, accurate and sensitive with a higher repeatability, which can be used in the quantitative determination of the total saponins from Flos Hostae Plantagineae.