中国KM小鼠亚群遗传变异的研究

应用电泳方法和Festing下颌骨形态分析法对北京、上海、长春三大地区的四个中国KM小鼠的主要群体展开调查,并与近年从美国引进Swiss来源的NIH小鼠群体进行比较.结果显示:1.中国KM与NIH小鼠群体在Es-3,Es-10,Got-2,Glo-1,Gpt-1,和Mpl-1座位的等位基因组成存在显著差异;两者间遗传距离为0.131±0.011,证实中国KM小鼠为非Swiss来源的另一亚种。2.KM小鼠各亚群间等位基因分布无明显差异,亚群间遗传距离为0.008～0.027,与群体封闭时间成正相关.3.对群体生化和下颌骨形态学数据聚类分析表明S:KM在四个KM亚群中为特殊的一类,提示不同地区KM亚群存在的遗传差异可能对实验重复性产生影响.

Study on Genetic Variants of Chinese KM Mouse Subcolonies

This paper presents the results of study on genetic variants of 4 KM subcolonies maintained in Beijing, Shanghai, Changchun and compares it with that of one NIH colony introduced from U.S.A. by methods of eletro-phoresis and mandible analysis, The results revealed that: (1) There are significant differences between KM and Swiss derivative colony NIH on alle-lic constitutions of Es-3, Es-10, Glo-1, Gpt-l, Got-2, and Mpi-1 loci and mandible shape. Genetic distance between NIH and KM subcolonies is 0.131, 0.011, which is a proof that KM mice is non-Swiss derivative subspecies. (2) The allelic constitutions of KM subcolonies is same and genetic distance is 0.008, 0.027 positively related with the time of colony closed. (3 ) The unique position of S:KM mouse was shown in phylogenetic diagram of 4 KM subcolonies from both biochemical and morphological data,which prompts that some variants existed in KM subcolonies may influence the repro-ductivity of experiment.