尾吊、噪声及其复合因素对大鼠免疫功能的影响

目的研究尾吊、噪声以及两种因素复合对大鼠免疫功能的影响。方法大鼠30°尾吊21 d、噪声2h及尾吊(21 d)+噪声(2 h)复合处理后,测定大鼠体重、胸腺重、脾重、白细胞及其分类,流式细胞术、Griess法分别测定T淋巴细胞亚群和血清NO含量。结果与对照组相比,尾吊组与尾吊+噪声复合应激组大鼠体重显著下降(P0.01),外周血白细胞数、中性粒细胞数及其百分比显著增加(P0.01),而淋巴细胞百分比显著下降(P0.05),尾吊+噪声复合应激组大鼠T淋巴细胞数和Th亚群数增加(P0.05),Th/Tc比显著升高(P0.01),各处理组大鼠血清NO含量均显著降低(P0.01)。结论尾吊明显影响大鼠体重、外周血免疫细胞含量和分布以及血清NO含量,噪声对尾吊大鼠淋巴细胞亚群的变化有协同效应。

Effects of Tail-suspension, Noise and Combination of Two Stresses on Immune Function of Rats

Objective To investigate the effect of tail-suspension, noise stress and combination of them on immune function in rats. Methods The rats were divided into four different groups, A; control; B; noise stress ( 2 h) ;C; tail-suspension (30° 21 d) ;D; tail-suspension added noise stress. The body weight, thymus weight and spleen weight, circulating leukocytes and its distribution , T lymphocyte subpopulations (with flow cytome-try) and NO level in serum (with Griess method) of rats were determined after exposed to 21 d tail-suspension , 2 h noise stress and combination of them. Results Compared with group A, the body weight were significantly decreased (P <0. 01 ) , the percent of lymphocytes were also reduced (P <0. 05) , but the numbers and percents of circulating leukocytes, neutrophils were markedly increased (P <0. 01) in group C and D. T lymphocyte, Th subsets (both P<0.05) and Th/Tc ratio (P<0.01) increased in group D. The NO level in serum was markedly reduced (P<0.01) in B, C and D groups. Conclusion Tail-suspension had significant effects on the body weight, circulating leukocyte and its distribution, NO level in serum of rats. Noise stress had synergistic effect with tail-suspension on lymphocyte subsets.