| 白花蛇舌草总黄酮的提取以及体外对肝癌细胞的作用 |
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| 引用本文： | 吴杨,周忆新,吴银生.白花蛇舌草总黄酮的提取以及体外对肝癌细胞的作用[J].抗感染药学,2008,5(3):150-152. |
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| 作者姓名： | 吴杨 周忆新 吴银生 |
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| 作者单位： | 1. 江苏省苏州惠氏制药公司,江苏,苏州,215000
2. 江苏省苏州药品检验所,江苏,苏州,215002 |
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| 摘 要： | 目的：提取白花蛇舌草总黄酮，并用MTT法检测其对HepG-2肿瘤细胞的增殖抑制作用。方法：用加热回流的方法提取白花蛇舌草中的总黄酮，标准曲线法测定其含量，然后作用于HepG-2肿瘤细胞，MTT法检测白花蛇舌草中的总黄酮对HepG-2肿瘤细胞生长抑制的影响。结果：成功提取并获得白花蛇舌草总黄酮，其含量约为15％，对人肝癌细胞HepG-2的生长增殖有抑制，48h后效果明显。结论：白花蛇舌草中的总黄酮对人肝癌细胞HepG-2有显著的抑制作用。
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| 关 键 词： | 白花蛇舌草 总黄酮 MTT法 HepG-2细胞 |
Extraction of flavone in Oidenlandia diffusa (Willd.) Roxb. And its effecton hepatocellular carcinoma cells in vitro |
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| WU Yang,ZHOU Yi-xin,WU Yin-sheng.Extraction of flavone in Oidenlandia diffusa (Willd.) Roxb. And its effecton hepatocellular carcinoma cells in vitro[J].Anti-infection Pharmacy,2008,5(3):150-152. |
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| Authors: | WU Yang ZHOU Yi-xin WU Yin-sheng |
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| Institution: | WU Yang, ZHOU Yi-xin, WU Yin-sheng(1.Wyeth Pharmaceutzcal Co., Ltd, Suzhou Jiangsu 215002, China; 2 . Jiangsu Suzhou Institute For Drug Control, Suzhou Jiangsu 215002, China) |
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| Abstract: | Objective:To extract flavone in Oldenlandia diffusa(willd.)Roxb.and to evaluate its effect on HepG-2 cell growth inhibition by MTT assay.Methods:To extract flavone in Oldenlandia diffusa(willd.)Roxb.by refluxing and to determine its content by Standard Curves Methods,and then to detect its effect on human HCC cell line (HepG-2)cell growth inhibition by MTT assay.Results:The flavone in Oldenlandia diffusa(willd.)Roxb.was extracted and obtained successfully.Its content was about 15% and it inhibited HepG-2 cell growth.Conclusion:The flavone in Oldenlandia diffusa(willd.)Roxb.can inhibit HepG-2 cell growth obviously. |
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| Keywords: | Oldenlandia diffusa(willd )Roxb FOD MTT assay HepG-2 cell |
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