川芎中川芎嗪和阿魏酸含量的毛细管电泳测定

目的：研究川芎中川芎嗪和阿魏酸的毛细管电泳分离分析的可行性。方法：通过用毛细管区带电泳分离、紫外检测模式研究内标、电泳缓冲液、进样方式、分离电压等对样品中川芎嗪和阿魏酸分离与定量的影响，优化实验条件。结果：以对硝基苯甲酸为内标，未涂层熔融石英毛细管(39.5 cm×50 μm ID，有效分离长度34.8 cm)为分离通道，30 mmol.L^-1硼砂缓冲液(pH 9.43)为电泳介质，34 kPa.s压力进样，17 kV分离电压，295 nm检测，川芎嗪和阿魏酸分别在7～423 μg.mL^-1和4～900 μg.mL^-1范围内可进行定量检测，回收率分别为100.9％±1.9％和99.8％±1.0％。结论：毛细管电泳法可用于川芎中川芎嗪和阿魏酸的同时分离分析。

DETERMINATION OF LIGUSTRAZIN AND FERULIC ACID IN CHUANXIONG RHIZOME BY CAPILLARY ELECTROPHORESIS

AIM:To investigate the feasibility of capillary zone electrophoresis (CZE) in the separation and determination of ligustrazin and ferulic acid in Chuanxiong. METHODS: Based on the effects of internal standard (p-nitrobenzoic acid), electrolyte, sampling method and running voltage, etc. on the quantitative assay of ligustrazin and ferulic acid in samples, the optimal conditions by CZE, UV detection were chosen. RESULTS: The experimental conditions are stated as follows: separation column: uncoated fused-silica capillary (39.5 cm×50 μm ID, 34.8 cm to UV detector); electrolyte: 30 mmol.L^-1 borax, pH 9.43; detection wavelength: 295 nm; sampling: 34 kPa.s; running voltage: 17 kV; temperature: 20℃. The linear ranges of determination for ligustrazin and ferulic acid are 7～423 μg.mL^-1 (γ=0.9995, RSD=0.61%～0.89%) and 4～900 μg.mL^-1 (γ=0.9992, RSD=0.22%～1.15%) respectively, and the recoveries of ligustrazin and ferulic acid are 100.9％±1.9％ (RSD=1.86%, n=6) and 99.8％±1.0％ (RSD=1.03%, n=6), respectively. CONCLUSION: CZE is a good method for the simultaneous separation and determination of ligustrazin and ferulic acid in Chuanxiong.