大鼠肝移植自然耐受模型移植术后Foxp3基因表达的变化

目的 探讨大鼠肝移植自然耐受模型移植术后Foxp3基因表达的变化及意义.方法 分别建立大鼠急性排斥(DA→LEW)、自然耐受(LEW→DA)、同基因组(DA→DA)肝移植模型,收集术后7、14、28d外周血、脾脏、肝脏标本,FCM检测标本淋巴细胞中CD4 CD25 Tr (regulatory T cell)比例变化,RT-PCR, Western blot分别检测肝脏内Foxp3基因及其scrufin蛋白的表达.结果 术后7d,各组外周血、脾脏、肝脏中CD4 CD25 Tr细胞比例无明显差异.术后14d自然耐受组脾脏、肝脏内CD4 CD25 Tr细胞比例明显高于同基因组(P<0.05),外周血中差异无统计学意义(P>0.05).术后28d自然耐受组与同基因组间无差异(P>0.05).术后7d,自然耐受组移植物Foxp3 mRNA水平明显高于排斥组和同基因组(P<0.05);术后14d,自然耐受组移植物Foxp3 mRNA水平明显高于同基因组(P<0.05);术后28d,自然耐受组与同基因组移植物Foxp3 mRNA表达无差异(P>0.05).自然耐受组与同基因术后14、28d scrufin蛋白条带明显强于7d,且自然耐受组表达强于同基因组.结论 大鼠肝移植术后1～2周内,移植肝内Foxp3基因表达可诱导机体对移植物产生免疫耐受.

Foxp3 gene expression in rat allograft liver transplantation tolerance model

Objective To investigate the expression of Foxp3 gene and the significance of the change in the tolerance model of rat allograft liver transplantation.Methods After rat models of tolerance and isogenic liver transplantation were established,blood, spleen and graft samples from each group were collected on the 7th, 14th and 28th post-transplantation day.The ratio of CD4+CD25+ T cells in the PBMC separating from blood, spleen and graft was compared.The expression of Foxp3 gene in the graft was detected by rea...