丹参药材水溶性成分含量的HPLC法分析

目的建立丹参药材水溶性成分中丹酚酸B、丹参素和原儿茶醛的含量测定方法。方法采用高效液相色谱法测定，色谱柱为Kromosil C18（4．6mm×250mm，5μm）；流动相I：甲醇-乙腈-甲酸-水（30：10：1：59），流动相Ⅱ：甲醇-冰醋酸-水（20：1：80）；流速：1．0mL／min；检测波长分别为286、280nm；柱温35℃。结果测定了10个不同产地的丹参药材，丹酚酸B含量在0.09％～6．81％之间，平均回收率为99.64％，RSD=1．09％；丹参素的含量在0．16％～0．68％之间，平均回收率为98.94％，RSD=1．49％；原儿茶醛的含量在0．01％～0.09％之间，平均回收率为98．96％，RSD=1．66％。结论本法简便、准确、重复性好，可作为丹参药材的含量测定标准。

Determination of Water Soluble Components in Salvia miltiorrhiza Bge. by HPLC

Objective To establish a method for determination of the water-soluble components of Salvianolic acid B, Danshensu and Protocatechuic aldehyde in Salvia miltiorrhiza Bge. Method Chromatography was performed on Kromosil C18 （4.6 mm×250 mm, 5 μm）. Mobile phase I was methanol-acetonitrile-formicacid-water （30 ： 10 ： 1 ： 59）, mobile phase Ⅱ was methanol-acetic acid-water （20 ： 1 ： 80）, the flow rate was 1.0 mL/min, the detection wavelength was set at 286 and 280 nm, column temperature was 35℃. Result Determined 10 different habitats Salvia miltiorrhiza Bge., the average content of Salvianolic acid B was 0.09%-6.81%, the average recovery was 99.64%, RSD= 1.09%. The average content of Danshensu was 0.16%-0.68%, the average recovery was 98.94%, RSD=1.49%. The average content of Protocatechuic aldehyde was 0.01%-0.09%, the average recovery was 98.96%, RSD= 1.66%. Conclusion The method is accurate and reliable with good reproducibility, and can be used as determination standard for Salvia miltiorrhiza Bge.