姜黄素调节AKT/PI3K活性抑制人膀胱癌T24细胞的增殖和迁移

研究姜黄素对膀胱癌T24细胞增殖和侵袭的影响及其作用机制。培养膀胱癌T24细胞,然后采用不同浓度的姜黄素进行干预。使用CCK8法检测姜黄素处理后T24细胞的存活率,Transwell实验检测其侵袭力。采用western blot检测处理前后T24细胞中基质金属蛋白酶2(MMP2)和MMP9的表达水平以及蛋白激酶B(AKT)和磷脂酰肌醇3-激酶(PI3K)磷酸化。CCK8结果表明姜黄素能够以浓度和时间依懒性抑制T24细胞的增殖。Transwell结果显示姜黄素能降低膀胱癌T24细胞的迁移能力。姜黄素也能降低T24细胞p-AKT和p-PI3K磷酸化以及MMP2和MMP9蛋白的表达,并且呈浓度依赖性。以上结果表明姜黄素通过抑制AKT/PI3K信号通路降低MMP2和MMP9的表达,从而抑制膀胱癌T24细胞的增殖和迁移。

Curcumin inhibits proliferation and migration of human bladder cancerT24 cell line by modulating AKT/PI3K signaling pathway

To study the effect of curcumin on the proliferation and invasion of bladder cancer T24 cells and its mechanism.The bladder cancer T24 cells were cultured and treated with different concentrations of Curcumin.The CCK8 assay was used to detect the inhibitory growth rate of the curcumin on T24 cells.The transwell matrix penetration assay was used to detect the cell migration capabilities.The expressions of MMP2(matrix metalloproteinase),MMP9,AKT,p-AKT,PI3K and p-PI3K protein were analyzed by Western blotting.CCK8 results show that curcumin can inhibit the proliferation of T24 cells in terms of concentration and time.Transwell results demonstrate that the intervention of curcumin can significantly reduce the migration of bladder cancer T24 cells.Curcumin intervention can reduce the expressions of p-AKT,p-PI3K,MMP2 and MMP9 in bladder cancer T24 cells in a dose-dependent manner.The higher the curcumin concentration was used,the lower expression of p-AKT,p-PI3K,MMP2 and MMP9 were observed in T24 cells.The results showed curcumin may down-regulate the expression of MMP2 and MMP9 by suppressing the AKT/PI3K signaling pathway,thereby inhibiting the proliferation and migration of bladder cancer T24 cell.