| 不同产地何首乌叶绿体psbA-trnH基因序列分析 |
| |
| 引用本文： | 张宏意,袁林林,饶秋红,李威,严寒静.不同产地何首乌叶绿体psbA-trnH基因序列分析[J].中草药,2018,49(5):1146-1149. |
| |
| 作者姓名： | 张宏意 袁林林 饶秋红 李威 严寒静 |
| |
| 作者单位： | 广东药科大学中药学院, 广东 广州 510006,广东药科大学中药学院, 广东 广州 510006,广东药科大学中药学院, 广东 广州 510006,广东药科大学生命科学与生物制药学院, 广东 广州 510006,广东药科大学中药学院, 广东 广州 510006 |
| |
| 基金项目： | 广东省中医药局科研项目（20163010，20182076）；大学生创新创业训练计划项目（201710573016）；广东省科技厅项目（2017A020213023） |
| |
| 摘 要： | 目的采用叶绿体基因psbA-trnH间隔区序列探讨不同产地何首乌的分子鉴定方法。方法分别提取7省区15个居群116份何首乌样品的总DNA,经PCR扩增psbA-trnH间隔区序列,纯化后测序,采用MEGA 6.06软件对序列进行分析。结果何首乌各居群间的遗传距离为0.001~0.187,最大似然法系统树中何首乌15个居群样品聚为2支。结论何首乌的遗传变异较显著,psbA-trnH序列可作为道地种源德庆种源与其他种源分子鉴定的依据。
|
| 关 键 词： | 何首乌 psbA-trnH基因 最大似然法系统树 |
| 收稿时间： | 2017/8/9 0:00:00 |
Sequence analysis of psbA-trnH gene in chloroplast of Polygonum multiflorum from different producing areas |
| |
| ZHANG Hong-yi,YUAN Lin-lin,RAO Qiu-hong,LI Wei and YAN Han-jing.Sequence analysis of psbA-trnH gene in chloroplast of Polygonum multiflorum from different producing areas[J].Chinese Traditional and Herbal Drugs,2018,49(5):1146-1149. |
| |
| Authors: | ZHANG Hong-yi YUAN Lin-lin RAO Qiu-hong LI Wei and YAN Han-jing |
| |
| Institution: | School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China,School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China,School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China,School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China and School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China |
| |
| Abstract: | Objective The molecular identification method of Polygonum multiflorum from different producing areas was explored by using the sequencing of intergenic region of chloroplast genes psbA-trnH. Methods A total of 116 samples of P. multiflorum were collected from 15 populations in seven provinces and autonomous regions. The total DNA was extracted and the sequences of psbA-trnH were amplified by PCR. The purified PCR products were sequenced and analyzed by MEGA 6.06 software. Results The genetic distances among the populations of P. multiflorum are 0.001-0.187. In the maximum likelihood phylogenetic tree, 15 populations of P. multiflorum were clustered into two blanches. Conclusion The genetic variation of P. multiflorum is significant and the psbA-trnH sequences of P. multiflorum can be used as germplasm source for molecular identification between Deqing regarded as geo-authentic habitat and other producing areas. |
| |
| Keywords: | Polygonum multiflorum Thunb psbA-trnH gene maximum likelihood phylogenetic tree |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中草药》浏览原始摘要信息 |
| 点击此处可从《中草药》下载免费的PDF全文 |
|
