应用组织芯片技术研究p27kip1和Skp2在星形胶质细胞瘤及增生中的表达和意义

目的:探讨p27kip1和Skp2在星形胶质细胞增生及星形胶质细胞瘤中的表达及其与肿瘤发生发展的关系。方法:利用组织芯片技术及PV6000通用型二步法免疫组化方法检测p27kip1和Skp2在正常脑组织,星形胶质细胞增生,低和高级别星形胶质细胞瘤中的表达。结果:正常脑组织中p27kip1阳性表达率91.7%,Skp2表达阴性;增生组中p27kip1和Skp2阳性表达率分别为86.4%、28.6%,与正常组比较,p27kip1无统计学意义;Skp2有统计学意义;低级别肿瘤组中二者阳性表达率分别为64.3%、46.7%,与增生组比较,p27kip1有统计学意义;Skp2无统计学意义;高级别肿瘤组中二者阳性表达率分别为42.6%、69.6%,与低级别肿瘤组比较,差异均有统计学意义;p27kip1和Skp2与组织学分级密切相关。结论:p27kip1有可能成为鉴别星形胶质细胞增生与低级别星形胶质细胞瘤的客观指标,且p27kip1和Skp2在星形胶质细胞瘤的发生发展过程中有重要作用。

The Expression and Significance of p27kip1, Skp2 in Astrocytomas and Astrocytes Proliferation: An Immunohistochemical Study Using Tissue Microarray Techniques

Objective: To detect the expression and significance of p27kip1?Skp2 in astrocytes proliferation and astrocytomas. Methods: Four tissue chips were constructed to contain 148 formalin-fixed , paraffin-embedded tissue samples which include 12 cases of normal brain tissues, 49 cases of astrocytes proliferation, 49 cases of low-grade astrocytomas and 50 cases of high-grade astrocytomas, 12 cases of normal brain tissues were used as controls. Immunohistochemical markers, including p27kip1?Skp2 were used on the tissue array sections by immunohistochemical staining methods. Results: The positive rate of p27kip1 was 91.7% in normal brain tissues, while that of Skp2 was 0; the positive rate of expression of p27kip1 and Skp2 in astrocytes proliferation was 86.4% and 28.6% respectively, compared to the control group. There was no significance about p27kip1 while there was a significance about Skp2 at 0.05 level, and the rate of expression in low-grade astrocytomas were 64.3%, 46.7% respectively, compared to the astrocytes proliferation group, the difference was significant at the 0.05 level about p27kip1 while there was no statistical significance about Skp2; in high-grade astrocytomas were 42.6%, 69.6% respectively, compared to the low-grade group, the difference was significant at the 0.05 level; the expression of p27kip1 and Skp2 had close correlation to histological grade. Conclusion: p27kip1 might be objective indexes to discriminate between astrocytes proliferation and low-grade astrocytomas, and p27kip1 and Skp2 play an important role in the progression of astrocytomas.