| 低氧特异性重组腺相关病毒VEGF165的构建与表达 |
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| 引用本文： | 李桂林,栗世方,窦万臣,张波,王欣,孔燕国,王任直. 低氧特异性重组腺相关病毒VEGF165的构建与表达[J]. 中国微侵袭神经外科杂志, 2005, 10(1): 23-25 |
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| 作者姓名： | 李桂林 栗世方 窦万臣 张波 王欣 孔燕国 王任直 |
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| 作者单位： | 1. 中国医学科学院中国协和医科大学北京协和医院神经外科,北京,100730
2. 中国协和医科大学生化系,北京,100730 |
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| 摘 要： | 目的构建VEGF165低氧启动rAAV载体,用于缺血性脑血管病的治疗.方法通过分子生物学的方法,构建VEGF165低氧启动rAAV载体,采用磷酸钙和氯仿-PEG8000/NaCl-氯仿法构建低氧启动rAAV病毒.通过体内外低氧诱导,验证VEGF165低氧启动rAAV对低氧的特异性.结果体内外实验证实,VEGF165低氧启动rAAV仅在低氧诱导后表达具有生物活性的VEGF蛋白,对低氧具有特异性.结论VEGF165低氧启动rAAV构建成功,可望用于缺血性疾病的基因治疗.
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| 关 键 词： | 重组腺相关病毒 低氧启动 血管内皮生长因子 |
| 文章编号： | 1009-122X(2005)01-0023-03 |
| 修稿时间： | 2004-12-28 |
Construction and express of VEGF165 hypoxia specific recombinant adeno-associated virus |
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| LI Guilin,LI shifang,DOU Wanchen,et al. Construction and express of VEGF165 hypoxia specific recombinant adeno-associated virus[J]. Chinese Journal of Minimally Invasive Neurosurgery, 2005, 10(1): 23-25 |
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| Authors: | LI Guilin LI shifang DOU Wanchen et al |
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| Affiliation: | LI Guilin,LI shifang,DOU Wanchen,et alDepartment of Neurosurgery,Peking Union Medical College Hospital,Chinese Academy of Medical Science & Peking Union Medical College,Beijing 100730,China |
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| Abstract: | Objective To construct VEGF165 hypoxia promoter rAAV for gene therapy of cerebral ischemia. Methods Molecular biochemistry methods were used to construct VEGF165 hypoxia promoter rAAV vector. VEGF165 hypoxia specific rAAV was produced through phosphorated calcium and chloroform-PEG8000/NaCl-chloroform method. rAAV protein was defined through SDS-PAGE protein electrophoresis. The specificity of VEGF165 hypoxia promoter rAAV to hypoxia was tested by experiment in vitro and in vivo. Result VEGF165 hypoxia promoter rAAV expressed VEGF165 only after inducing by hypoxia environment. Conclusion VEGF165 hypoxia specific recombinant adeno-associated virus was constructed successfully, and it may be applied to treat the cerebral ischemia. |
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| Keywords: | recombinant adeno-associated virus hypoxia promoter VEGF |
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