3种梅毒血清学筛选试验的方法学评价

目的:评价3种不同的梅毒血清学筛选试验方法和各项试验之间的相互关系及应用价值。方法:以酶联免疫吸附试验(TP-ELISA)检测门诊和住院患者标本共12 960例。TP-ELISA阳性者再做梅毒螺旋体明胶颗粒凝集试验(TPPA)及甲苯胺红不加热血清学试验(TRUST),结果进行统计学处理。结果:TP-ELISA筛出阳性334例,TPPA确证277例,TRUST阳性的98例。结论:TP-ELISA、TPPA两种检测方法有较高的灵敏度和特异性。TRUST的灵敏度比ELISA、TPPA差且特异性也不如二者,但可用TRUST法检测判断是否为现症患者,以及观察梅毒疗效及判断预后。TP-ELISA法是大批量梅毒筛查的理想方法。

Three Kinds of Syphilis Serology Screening Test of the Method of Evaluation

Objective:To evaluate the three types of Syphilis specific serum screening test methods and all test the relationship between the value.Methods:By Enzyme-linked immunosorbent assay(TP-ELISA) detect outpatient and inpatient samples a total of 12 960 cases.TP-ELISA positive again the Syphilis spirochete gelatin particle agglutination test(TPPA) and the toluidine red unheated serological test(TRUST),the results were analyzed statistically.Results:TP-ELISA to screen positive in 334 cases,277 cases of TPPA conclusive evidence of positive and TRUST positive in 98 cases.Conclusion:Both the two detection methods of TP-ELISA and TPPA have high sensitivity and specificity.The sensitivity and specificity of TRUST are poorer than ELISA and TPPA,but can TRUST method testing to determine whether the current disease patients,as well as observation of syphilis efficacy and prognosis.TP-ELISA is the ideal way to large quantities of syphilis.