| OGP对NIH3T3细胞作用机理的研究 |
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| 引用本文： | 王晓峰,刘智慧,邵云潮,崔大敷,陈统一. OGP对NIH3T3细胞作用机理的研究[J]. 中国临床医学, 2005, 12(3): 480-481 |
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| 作者姓名： | 王晓峰 刘智慧 邵云潮 崔大敷 陈统一 |
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| 作者单位： | 1. 复旦大学附属中山医院骨科,上海,200032
2. 中国科学院生物化学所,上海,200032 |
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| 摘 要： | 目的：研究成骨生长肽(OGP)对NIH3T3细胞的作用机理。方法：细胞计数法测定不同浓度的OGP14肽及5肽对NIH3T3细胞的促增殖作用，以流式细胞仪测定细胞膜上OGP的表达。观察0GP抗体对OGP促增殖作用的影响。结果：OGP14肽及5肽在10^-11mol／L时对NIH3T3细胞有促增殖作用，同时细胞膜上OGP表达增加；OGP抗体可抑制OGP的促增殖作用，同时使细胞膜上OGP表达下降。结论：OGP14肽及5肽可以通过对OGP的正反馈来调节NIH3T3细胞的增殖。
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| 关 键 词： | 成骨生长肽（OGP） 流式细胞（FCM） OGP抗体 |
| 文章编号： | 1008-6358(2005)03-0480-02 |
The Mechanism of the Osteogenic Growth Peptide on NIH3T3 Cells |
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| WANG Xiaofen LIU Zhihui SHAO Yunchao,.et al.. The Mechanism of the Osteogenic Growth Peptide on NIH3T3 Cells[J]. Chinese Journal Of Clinical Medicine, 2005, 12(3): 480-481 |
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| Authors: | WANG Xiaofen LIU Zhihui SHAO Yunchao .et al. |
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| Abstract: | Objective: To study the mechanism of the osteogenic growth peptide(OGP) effects on NIH3T3 cells. Methods: We used cell counts to test the mitogenic action on NIH3T3 cells in different dilutions of OGP and OGP(10-14) ,and tested the variable expressions of OGP on cell membrane through flow cytometry (FCM), then we used cell counts to survey the mitogenic activity of OGP changed by anti-OGP. Results: OGP and OGP(10-14) could stimulate the proliferation of NIH3T3 cells in the dilution of 10-11 mol/L, and could down-regulate the expression of OGP on cell membrane. Conclusion: OGP and OGP(10-14) could regulate the proliferation of the NIH3T3 cells through the mechanism of positive feedback. |
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| Keywords: | Osteogenic growth peptide(OGP) Flow cytometry (FCM) Anti-OGP |
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