ACE基因多态性与2型糖尿病肾病的相关性研究

目的 探讨血管紧张素Ⅰ转换酶(ACE)基因的插入／缺失多态性与2型糖尿病(type 2 diabetes mellitus，T2DM)患合并肾病的关系。方法采用聚合酶链反应技术检测109例T2DM患(其中合并肾病患37例，未发生肾病患72例)和260例健康对照组ACE基因插入／缺失多态性。结果糖尿病肾病患的DD基因型频率和D等位基因频率有高于无肾病糖尿病患组的趋势，其频率差异接近显性水准(75．7％vs55．6％，P=0．070；87．8％vs77．1％，P=0．057)。DD基因型糖尿病患合并肾病的频率高于其他基因型，差异有统计学意义(41．2％vs22．0％，P=0．040)。对糖尿病病程与ACE基因多态性的交互作用分析发现，DD基因型与5年以上病程存在交互作用(OR=3．75，95％CI；1．019～13．795)。结论ACE基因的DD基因型可增高T2DM患并发肾病的危险性，并且与糖尿病病程有交互作用。

Study on the relationship between ACE gene polymorphism and type 2 diabetes with nephropathy

Objective To explore the association between gene polymorphisms of angiotensin I converting enzyme(ACE) and diabetic nephropahty(DN). Methods Angiotensin I converting enzyme(ACE) genotypes were investigated with PCR techniques in 109 cases of T2DM and 260 controls. Results The D allele frequency and DD genotype frequency were higher in diabetic nephropathy, but no significant differences were found between diabetic nephropathy and that non diabetic nephropathy (75.7% vs 55.6%, P=0.070; 87.8% vs 77.1%, P=0.057). The frequency of nephropathy were higher in diabetic with DD genotype (41.2% vs 22.0%,P=0.040). Interaction was found in DD genotype with duration more than 5 years (OR=3.75,95%CI;1.019~13.795). Conclusion It is suggested that ACE gene I/D polymorphism is a risk factor for duiabetic nephropathy in China and it cooperated with duration of T2DM.