大鼠肝癌自发肺转移裸鼠模型的建立

目的　建立大鼠肝癌细胞系自发性肺转移裸鼠模型。方法　大鼠肝癌细胞系C5F皮下接种 4周龄雄性和 7周龄雌性BALB/CA裸鼠 ,观察皮下成瘤及肺部和腹腔脏器的大体转移灶形成情况 ,取可能转移的器官作组织学检查 ,肺组织连续切片并计数镜下转移灶。结果　裸鼠皮下成瘤率 10 /10 ,雌性裸鼠中肺表面肉眼可见明显转移灶 ,转移率为 6/6,肺表面大体转移灶中位数45 ,镜下转移灶中位数 75 5个 /裸鼠 ,腹腔及其脏器均未见转移灶。雄性裸鼠未见大体和镜下转移灶。结论　该细胞系在雌性BALB/cA裸鼠中能充分表达自发转移潜能 ,肺脏是其优势转移器官。成功建立的大鼠肝癌细胞自发肺转移裸鼠模型为肝癌转移抑制基因的染色体功能定位研究提供了适宜的动物模型。

The establishment of a spontaneous metastatic model of rat liver cancer in nude mice

Objective To establish a spontaneous metastatic model of rat liver cancer in nude mice.Methods The rat liver cancer cell line C5F was subcutaneously implanted in 4-week-old male and 7-week-old female BALB/cA nude mice respectively.Subcutaneous tumor formation was observed by measuring the MTD weekly.6-7 weeks thereafter,the moribund mice were sacrificed and macroscopic metastases were checked by autopsy.Serial section of the fixed lung tissue was done to grade and count lung metastases.Histological examination was performed for suspicious organs.Results The rate for the subcutaneous tumor formation was 100% (10/10).In the female nude mice,100% (6/6) overt metastases were observed.The median numbers of gross and microscopic metastatic foci were 45 and 755 per nude mouse respectively.Metastases were not found in abdomen and its organs.In contrast,neither macroscopic nor microscopic metastasis was observed in male nude mice.Conclusion This cell line can optimally demonstrate its spontaneous metastatic potential in female BALB/cA nude mice,with the lungs being the preferential target organ.The spontaneous metastatic behavior of C5F in BALB/cA nude mice could be used as a model for functional localization of metastatic suppressor genes for liver cancer on human chromosomes.