压力蒸汽灭菌用BS-Y型指示剂的研制

<正> 为改进压力蒸汽灭菌监测方法,研制成由嗜热脂肪杆菌芽胞、pH指示培养基与温度指示剂等3部分组成的BS-Y型指示剂。该指示剂用于监测压力蒸汽灭菌,可直接了解是否达到规定温度(121℃),此外经培养后还可根据培养基颜色变化进一步确定灭菌的效果。现将研制结果报告于下。

DEVELOPMENT OF INDICATOR TYPE BS-Y FOR AUTOCLAVING

The indicator type BS-Y for monitoring autoclaving was developed in laboratory. It consists of a tube containing benzoic acid which can indicate temperature and a biological indicator made of spores of Bacillus stearothermophilus. The two indicators are sealed in an ampoule containing culture medium. By using it, one can know the temperature attained during sterilization and whether the sterilization requirement is fulfilled or not after culturing the indicator under 55℃ for 48 hr. Modified Cook agar culture medium used for culturing spores of Bacillus stearothermophilus. The D value of the biological indicator is 5±1 min at 121℃ and its kill time is 20 min