连翘的组织培养和连翘苷形成的研究

目的 :研究影响连翘愈伤组织生长和连翘苷生物合成的培养条件。方法 :选用不同的培养基和不同的光照条件 ,测定愈伤组织生长干重和连翘苷含量。结果和结论 :连翘细胞生长周期为 28d ,采用MS基本培养基 ,附加激素为 1mg·L^-12 ,4-D ,0.5mg·L^-1 KT ,0.5mg·L^{-16-BA ,在光照条件下可有效促进细胞生长和连翘苷的生物合成。}

Studies on Callus Growth and Phillyrin Accumulation of Forsythia suspensa

Objective: To investigate the effects of physical and chemical factors on callus growth and phillyrin contents of F. suspensa. Method: The cell growth index and phyllirin yield in different culture condition such as different plant hormones mixed, mediums, light and dark were compared. HPLC was used to examine phillyrin contents. Result and Conclusion: Growth cycle of cells is twenty eight days. During the course of callus growth, the processes of phillyrin biosynthesis were parallel with the cell growth.The optimum medium is MS.The optimum hormones concentrations are 1 mg·L^-12,4-D,0.5mg·L-16-BA and 0.5 mg·^-1KT.The cell culture in light is more suitable than in dark.