以恶性疟原虫裂殖子表面蛋白1的17区片段基因为基础的复合核酸疫苗诱导小鼠体液免疫反应的研究

目的: 检测以恶性疟原虫裂殖子表面蛋白1 的17 区片段基因为基础的复合核酸疫苗VR1012/TPA/HG-MSP1-17(分泌性)和VR1012/HG-MSP1-17(非分泌性)诱导小鼠的体液免疫反应。方法: 分别用分泌性与非分泌性核酸疫苗肌注免疫小鼠。用间接ELISA法测定小鼠血清的特异性抗体。结果: 每只小鼠经3 次100 μg/100μl非分泌性核酸疫苗免疫后, BALB/c小鼠和C57BL/6 小鼠均产生明显的抗HG和抗真菌表达的MSP1 17 区蛋白(YMSP119 )抗体。但总体抗体水平不高。BALB/c小鼠经3 次200 μg/100 μl非分泌性核酸疫苗免疫后, 产生较高的抗HG抗体, 但抗MSP1-17 的抗体无明显变化。经3 次200 μg/100 μl分泌性核酸疫苗免疫后, 只产生较低的抗HG抗体。结论: 非分泌性核酸疫苗较分泌性核酸疫苗具有更强的免疫原性

HUMORAL IMMUNE RESPONSE IN MICE TO HYBRID NUCLEIC ACID VACCINES CONTAINING PLASMODIUM FALCIPARUM MEROZOITE SURFACE PROTEIN 1 BLOCK 17 BASED GENE *

AIM: To analyse the humoral immune response in mice to nucleic acid vaccines (VR1012/HG-MSP1-17 for intracellular expression or VR1012/TPA/HG-MSP-17 for secretion) containing Plasmodium falciparum merozoite surface protein 1 (MSP1) 17 block gene and gene fragment of several T cell epitopes from MSA1, MSA2, RESA, IL-1 and TT. METHODS: BALB/c or C57BL/6 mice received three times intramuscular immunization with 200 micrograms/100 microliters or 100 micrograms/100 microliters of VR1012/HG-MSP1-17 or VR1012/TPA/HG-MSP1-17 per mouse each time. Anti-HG or anti-MSP1-17 antibodies were monitored by indirect ELISA. RESULTS: BALB/c and C57BL/6 mice immunized with 100 micrograms/100 microliters of VR1012/HG-MSP1-17 per mouse raised significantly anti-HG and anti-MSP1-17 antibodies, but the levels of antibodies were not high. BALB/c mice immunized with 200 micrograms/100 microliters of VR1012/HG-MSP1-17 per mouse raised higher anti-HG antibodies but not anti-MSP1-17 antibodies. BALB/c mice immunized with 200 micrograms/100 microliters of VR1012/TPA/HG-MSP1-17 per mouse raised low level of anti-HG antibodies only. CONCLUSION: VR1012/HG-MSP1-17 is more immunogenic than VR1012/TPA/HG-MSP1-17.