白芷中香豆精类成分的反相高效液相色谱测定

本文用反相高效液相色谱法分离测定了生药白芷中三种主要有效成分;氧化前胡素、欧前胡素和异欧前胡素。在键合相ODS柱上,以甲醇—水(70:30)为流动相,紫外检测。对不同产地、采收期、种类、干燥方法、贮存方法、加工方法及不同部位等的42种白芷进行了分析,样品中各组分分离良好,用微处理机按外标法定量,变异系数≤3.33%,回收率97.91～101.36%。本方法与薄层光密度法分析结果一致。

REVERSE-PHASE HPLC DETERMINATION OF COUMARINS IN THE TRADITIONAL CHINESE DRUG BAI-ZHI (ANGELICA DAHURICA FORMA BAI-ZHI)

A reversed phase HPLC procedure has been developed for the separation and determination of oxypeucedanin (I), imperatorin (II) and isoimperatorin (III) in Bai-zhi simultaneously. I, II and III were extracted from Bai-zhi by using ether. The solution was evaporated to dryness and the residue was redissolved in 25 ml of ethyl acetate and 10 microliters of the solution were analysed on Waters mu Bondapak C13 column (300 x 3.9 mm ID) using methanol-water (70:30) as the mobile phase and detecting at 254 nm. The results were calculated by external standard method on the basis of peak area. The average recoveries of the standards added to the sample were 101.36, 100.54 and 97.91% for I, II and III respectively, and coefficients of variation were less than or equal to 3.33%. Analytical results by HPLC method are in agreement with those by TLC method. This method is rapid, reproducible and accurate and has been applied to analyse the contents of three components in 42 samples of angelica. The results of fourteen samples were given.