雷帕霉素抑制兔眼后发性白内障的实验研究

目的：探讨雷帕霉素（Rapamycin,RAPA）抑制兔眼后发性白内障发生的有效性及可行性。方法：雄性新西兰大白兔12只，24眼随机分为空白对照组，RAPA 7.5、15和30?ng/ml组,4组均行超声乳化透明晶状体吸除术；实验组灌注液中分别加入不同浓度的RAPA。术后不同时间裂隙灯观察兔眼角膜、房水及晶状体后囊膜混浊情况；3个月后处死动物，分别对4组兔眼行组织病理学及电镜检查。结果：术后12周，RAPA15?ng/ml组和30?ng/ml组后囊混浊发生率较对照组明显减少（P＜0.05）；各组术后裂隙灯下角膜混浊度无明显差异。结论：浓度为15～30?ng/ml的RAPA灌注液可有效抑制兔眼后发性白内障的发生且对角膜无明显的毒性作用。

Rapamycin inhibits the pathogenesis of posterior capsule opacification: experimental study on rabbit

To explore the role of rapamycin in the pathogenesis of posterior capsule opacification(PCO). Methods: Twelve New Zealand rabbits (24 eyes) were performed phacoemulsification and randomly divided into 4 groups. The irrigation solution of 7.5?ng/ml, 15?ng/ml and 30?ng/ml rapamycin was used in 3 experimental groups respectively. The posterior capsule and cornea were observed with slit lamp microscope in different times, then 3 months after the rabbits were killed, the histopathological changes and transmission electronic microscope examination were measured. Results: The incidence of PCO was significantly decreased in the 15?ng/ml and 30?ng/ml rapamycin groups compared with the control group in the 12th week after the operation (P＜0.05). Conclusion： Rapamycin can inhibit the PCO formation without obvious toxicity.