丁酸钠对人口腔鳞状细胞癌Tca8113细胞增殖及p27Kip1蛋白表达的影响

目的 通过观察组蛋白去乙酰化酶抑制剂丁酸钠对人口腔鳞状细胞癌Tca8113细胞的增殖影响及p27Kip1蛋白表达改变,探讨丁酸钠调控人口腔癌细胞增殖的分子机制.方法 人口腔鳞状细胞癌Tca8113细胞经不同浓度丁酸钠0 mmol/L(空白对照组),2、4、6、8 mmol/L(4个实验组)]作用后,甲基噻唑基四唑(MTT)法观察细胞增殖情况,流式细胞仪分析细胞周期分布,免疫组化检测p27Kip1蛋白的表达.结果 丁酸钠能够呈时间剂量依赖性抑制Tca8113细胞的增殖,丁酸钠处理后的Tca8113细胞出现了凋亡形态变化;细胞周期阻滞于G0～G1期,丁酸钠2 mmol/L组G0～G1期达(63.2±2.4)%,4 mmol/L组G0～G1期达(77.2±3.8)%,空白对照组G0～G1期达(48.1±2.4)%,P＜0.05;p27Kip1蛋白表达水平明显上调.结论 丁酸钠能够抑制人口腔癌细胞的增殖,该作用可能与p27KiP1蛋白表达上调及G0～G1期细胞周期阻滞有关.

Effects of sodium butyrate on proliferation of human oral squamous carcinoma cell line and expression of p27Kip1

Objective To investigate the effect of sodium butyrate (NaB) on inhibition of human oral squamous carcinoma cell line. Methods Human oral squamous carcinoma cell line Tca8113 was treated with different concentration of NaB. Light microscope was used to observe the morphological changes of the carcinoma cells. The cell cycle was analyzed by flow cytometry. Expression of p27Kip1 was determined with immunocytochemical assay. ResultsNaB significantly inhibited the proliferation of Tca8113 in a timeand dose-dependent manner. Tca8113 cells treated with NaB was arrested in Go-G1 phase. The fraction of cells in G0-G1 were (63.2 ± 2.4) % and (77.2 ± 3.8) % after treated with NaB at the concentration of 2 and 4 mmol/L alternatively, whereas (48.1 ±2. 4)% in Go-G1 phase were observed in control group(P ＜0. 05). The expression of p27Kip1 was markedly up-regulated after being treated with NaB. Conclusions NaB treatment can inhibit the growth of oral squamous carcinoma cell line in vitro and induce cell cycle arrest, which might be associated with the increased expression of p27Kip1 protein.