PTEN和p16基因蛋白在胆管癌和胆囊癌中表达的研究

目的探讨PTEN和p16抑癌基因在胆管癌和胆囊癌组织中的阳性表达及意义。方法用S-P免疫组化法测定PTEN、p16基因蛋白在63例胆管癌及胆囊癌石蜡标本的阳性表达率。结果胆管癌PTEN和p16阳性表达为42.4%和36.3%,低于胆囊癌的43.3%和46.6%(P>0.05);胆囊癌组低分化组p16表达明显低于高、中分化组(P<0.05);PTEN表达与分化程度差异无显著性(P>0.05),结论p16和PTEN基因在胆道恶性肿瘤组织中呈低表达,表明胆道恶性肿瘤的发生及发展有多基因变异或缺失。

The study of difference expression with PTEN and p16 proteins in gallbladder carcinoma and cholangiocarcinoma

Objective To study the difference expression rate of suppressor gene p16 and PTEN (phosphatase and tensun homologdeleted on chromosome 10) in gallbladder cancer and extraheptic bile duct carcinoma. Methods Selection paraffin sample with 30 cases gallbladder cancer and 33 cases extrahepatic bile duct cancer were studied by S- P immunohistochemical technique. Results Total expression rate of PTEN and p16 proteins showed lower level in cancer of bile duct system, PTEN expression rate in bileduct cancer is lower than in gallbladder cancer (P <0.05). In gallbladder cancer, the p16 and PTEN protein has significant difference expression rate in well- differentiated carcinoma and poorly- differentiated carcinoma (P <0.05). Conclusions There is a higher incidence of negative expression of PTEN protein and p16 protein in bile duct system carcinoma, which indicated PTEN and p16 gene inactivation may play an important role in the genesis and development of bile duct system carcinoma.