| 阿霉素纳米粒对白血病多药耐药细胞株K562/ADR耐药性的逆转作用 |
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| 引用本文： | 王良才,柯红. 阿霉素纳米粒对白血病多药耐药细胞株K562/ADR耐药性的逆转作用[J]. 中国医院药学杂志, 2004, 24(8): 468-470 |
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| 作者姓名： | 王良才 柯红 |
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| 作者单位： | 宜昌市葛洲坝中心医院,湖北,宜昌,443002 |
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| 摘 要： | 目的:研究阿霉素纳米粒对人白血病多药耐药细胞株K562/ADR耐药性的逆转作用.方法:采用MTT法测定药物的体外杀伤作用,应用流式细胞术测定细胞内药物浓度.结果:阿霉素纳米粒和阿霉素对K562细胞细胞毒作用相似,K562/ADR对阿霉素纳米粒较阿霉素敏感2.63倍,细胞内阿霉素浓度显著增加可能是关键因素.结论:阿霉素纳米粒通过增加耐药细胞内阿霉素浓度有效逆转多药耐药.
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| 关 键 词： | 阿霉素 聚氰基丙烯酸异丁酯 纳米粒 多药耐药 逆转 |
| 文章编号： | 1001-5213(2004)08-0468-03 |
| 修稿时间： | 2003-11-12 |
Reversal effect of adriamycin-loaded nanoparticles on human leukemia multidrug-resisitant cell line K562/ADR |
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| WANG Liang-Cai,KE Hong. Reversal effect of adriamycin-loaded nanoparticles on human leukemia multidrug-resisitant cell line K562/ADR[J]. Chinese Journal of Hospital Pharmacy, 2004, 24(8): 468-470 |
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| Authors: | WANG Liang-Cai KE Hong |
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| Abstract: | OBJECTIVE To investigate reversal effect on human leukemia multidrug-resistant cell line K562/ADR by adriamycin-loaded nanoparticles.METHODS The reversal effect of the drugs on multidrug-resistant cell line were determin ed by MTT method.Intracellular accumulation of ADR was studied by flow cytometr y.RESULTS The cytotoxicity of adriamycin-loaded nanoparticles and free adriamycin was i dentical in K562 cells,but the chemosensitivity of K562/ADR cells to adriamycin -loaded nanoparticles was increased to 2.63 times compared with that of K56 2/ADR cells to free adriamycin.The increased sensitivity of K562/ADR cells to ad riamycin-loaded nanoparticles was due to a remarkable increasing intracellular accumulation of adriamycin.CONCLUSION Adriamycin-loaded nanoparticles reverse MDR effectively by increasing intracell ular accumulation of adriamycin in K562/ADR cells. |
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| Keywords: | Adriamycin Polyisobutylcyanoacrylate Nanoparticles Multidrug resistance Reversal |
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