分泌性中耳炎渗出液的细胞因子检测

目的 :探讨分泌性中耳炎渗出液中细胞因子的水平及其炎症的调节机制。方法 :用 EL ISA法检测本病患耳的渗出液中肿瘤坏死因子 -α(TNF-α)和可溶性肿瘤坏死因子受体 (TNFsol R )水平 ,分析其与临床指标间的关系。结果 :渗出液中 TNF- α和 TNFsol R 的检出率均为 10 0 % ;TNF- α值为 (3.42± 2 .2 5 ) ng/ g总蛋白 ,TNFsol R 为 (4 0 5 .80± 2 16 .5 3) ng/ g总蛋白 ,TNFsol R 指数为 (15 4.18± 90 .45 ) U;多次发病的患者 TNFsol R 和 TNFsol R 指数均降低 (P <0 .0 5 )。结论 :细胞因子 TNF- α和 TNFsol R 存在于所有分泌性中耳炎渗出液中 ,是此炎症反应的重要递质。复发的分泌性中耳炎渗出液中 TNFsol R 和 TNFsol R 指数明显降低 (P <0 .0 5 ) ,而 TNF- α无明显变化。

Cytokine expression in effusions of secretory otitis media

OBJECTIVE: To assess the cytokine level in effusions of secretary otitis media and it's relationship with clinical index. To further study the inflammatory mechanism of otitis media with effusion. METHOD: The concentration of TNF-alpha and TNFsolR II was quantitatively determined using enzyme-linked immunosorbent assay and it was correlated with clinical index in otitis media with effusion using stepwise regression analysis. RESULT: TNF-alpha and TNFsolR II was found in 100% effusions. Mean values of TNF-alpha were (3.42 +/- 2.25) ng/g total protein. Mean values of TNFsolR II were (405.80 +/- 216.53) ng/g total protein. Mean values of TNFsolR II index was (154.18 +/- 90.45) U. There was a significant decrease of TNFsolR II and TNFsolR II index in patients who recurred (P < 0.05). CONCLUSION: Cytokine TNF-alpha and TNFsolR II are important inflammatory medium which exist in all otitis media effusions. The decrease of the level of TNFsolR II and TNFsolR II index present significantly in middle ear effusions with recurrence, but there is no any relation of TNF-alpha in middle ear effusions with recurrence.