PCR检测慢性胃炎患儿胃液中幽门螺杆菌DNA

目的检测慢性胃炎儿童胃液中幽门螺杆菌DNA，探讨其在临床诊断中的实用价值。方法应用PCR检测胃液中幽门螺旋菌DNA，应用Warthin-Starry银染色检测胃粘膜组织中的幽门螺旋菌。结果胃炎组与非胃炎组比较，敏感性为75.0％，特异性为96．2％，两组有显著性差异（P＜0．01）。PCR与Warthin－Starry银染色比较，PCR优于Warthin—Starry银染色法。结论PCR检测患儿胃液中的幽门螺旋菌DNA，具有敏感、快速、特异、高效等优点，是基因水平上检测幽门螺旋菌一项新技术，用于慢性胃炎的早期诊断，具有较好的实用价值，值得推广应用。

Detection of helicobactr pylori DNA with polymerase chain reaction in gastric juice of children

Objectives To determine helicobactr pylori DNA in gastric juice of the chikl patients with chronic gastritis and to search the application value of clinical diagnosis.Methods The helicobactr pylori DNA in gustric juice and the helicobactr pylori in gastric mucosa tissue were determined by polymerase chain reaction (PCR) and Warthin-Starry stain.Resul ts Compared with nongastritis, the sensitivity and specificity of the patients with gustritis were 75. 0% and 96. 2%.There was significant difference between them (P<0.01). Compared with Warthin-Starry stain,PCR is better.Conclusions It is sensitive,quick and specific that PCR is used to detect helicobactr pylori DNA. It is a ncw technique for the detection of helicobactr pylori in gene level. There is important value in the early diagnosis of chronic gastritis.