| 用多聚酶链反应检测患者丙型肝炎病毒RNA |
| |
| 引用本文： | 徐德忠 阎小君. 用多聚酶链反应检测患者丙型肝炎病毒RNA[J]. 医学争鸣, 1992, 13(4): 258-260 |
| |
| 作者姓名： | 徐德忠 阎小君 |
| |
| 作者单位： | 第四军医大学流行病学教研室(徐德忠，肖乐义)，第四军医大学生物化学教研室(阎小君)，第四军医大学生物化学教研室(苏成芝) |
| |
| 摘 要： |
|
| 关 键 词： | 聚合酶链反应 RNA 丙肝病毒 |
Preliminary study on detection for hepatitis C virus RNA by PCR in patients |
| |
| XU De-Zhong YAN Xiao-Jun,XIAO Le-Yi and SU Cheng-Zhi. Preliminary study on detection for hepatitis C virus RNA by PCR in patients[J]. Negative, 1992, 13(4): 258-260 |
| |
| Authors: | XU De-Zhong YAN Xiao-Jun XIAO Le-Yi SU Cheng-Zhi |
| |
| Affiliation: | XU De-Zhong YAN Xiao-Jun,XIAO Le-Yi and SU Cheng-Zhi Department of Epidemiology Department of Biochemistry |
| |
| Abstract: | Sera from 32 patients with liver disease or hematonosis and 5 donors with elevation of alanine amino transferase (ALT) were tested for HCV DNA by two-temperature-point PCR. It was found that a clear band in the positive sample could be seen on gel. The positive rate of HCV RNA was 48.6% in 37 cases. All of 5 cases with non-A non-B post-transfusion hepatitis and 3 cases with acute non-A non-B hepatitis were HCV RNA positive. A high positive rate (4/5) of HCV RNA was also found among patients with chronic hepatitis or cirrhosis. These suggest that hepatitis C predominates in various types of non-A non-B hepatitis in China. It was worthy of note that the proportion of HCV carriers in the patients with hematonosis and the donors with elevation of ALT was 2/6 and 1/5, respectively. The findings suggest that the PCR is a specific and sensitive method for diagnosis of hepatitis C. |
| |
| Keywords: | polymerase chain reaction hepatitis C virus RNA |
| 本文献已被 CNKI 维普 等数据库收录！ |
