Upregulation of the Long Non-coding RNA PlncRNA-1 Promotes Esophageal Squamous Carcinoma Cell Proliferation and Correlates with Advanced Clinical Stage |
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Authors: | Chun-Mei Wang Qing-Quan Wu Su-Qing Li Fang-Jun Chen Lei Tuo Hai-Wei Xie Yu-Suo Tong Lv Ji Guo-Zhi Zhou Gang Cao Ming Wu Jin Lv Wei-Hong Shi Xiu-Feng Cao |
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Affiliation: | 1. Department of Surgical Oncology, Affiliated Nanjing Hospital of Nanjing Medical University, Nanjing, Jiangsu, China 3. Huaian First Hospital, Nanjing Medical University, Huaian, Jiangsu, China 2. Department of Oncology, Changzhou Second Hospital Affiliated to Nanjing Medical University, Changzhou, Jiangsu, China 4. Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, Jiangsu, China 5. Department of Medical Technology, Yancheng Health Vocational, Yancheng, Jiangsu, China
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Abstract: | Background Recent studies revealed that long noncoding RNAs (lncRNAs) play critical regulatory roles in cancer biology. PlncRNA-1 is one of lncRNAs that is associated with cell apoptosis and proliferation of prostate cancer. Aim This study aimed to assess the potential role of PlncRNA-1 in the pathogenesis of esophageal squamous cell carcinoma (ESCC). Materials and Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression level of PlncRNA-1 in 73 pairs of ESCC and their matched normal tissues. The correlation of PlncRNA-1 with clinicopathological features and clinical stages was also analyzed. Cancer cell proliferation and apoptosis were assessed following knock-down of PlncRNA-1 by MTT, colony formation assay, and flow cytometry. Results The expression of PlncRNA-1 was significantly higher in human ESCC compared with the adjacent noncancerous tissues (69.8 %, p < 0.05), and the high level of PlncRNA-1 expression was significantly correlated with advanced clinical stage (p < 0.01) and lymph node metastasis (p < 0.05). Furthermore, knockdown of PlncRNA-1 reduced cell proliferation and increased the apoptosis in vitro. Conclusions PlncRNA-1 plays an important role in ESCC cell proliferation. Overexpression of PlncRNA-1 is correlated with advanced tumor stage and lymph node metastasis, and may serve as a potential prognostic marker and therapeutic target for ESCC. |
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