血瘀型非酒精性脂肪性肝病病证结合模型的建立和评价

目的:建立血瘀型非酒精性脂肪性肝病(NAFLD)病证结合模型。方法:70只大鼠随机分正常组10只、NAFLD组30只及血瘀型NAFLD组30只,正常组以大鼠标准饲料喂养,其余两组以高脂饲料喂养,同时,血瘀型NAFLD组联合应用去甲肾上腺素(NE)加小牛血清白蛋白(BSA)进行复合造模,在4、8、12周末3个时相点处理大鼠。观察大鼠血瘀体征,DRT4激光多普勒血流测定仪检测皮肤微循环,全自动血液流变快测仪检测血液流变学,全自动生化仪检测血脂及血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)水平,HE染色观察肝脏脂肪变和炎症程度。结果:血瘀型NAFLD大鼠出现舌下静脉曲张、舌质青紫有瘀斑、眼球暗红、尾部瘀斑等血瘀证候以及皮肤微循环障碍、血液流变学异常、血脂紊乱、肝组织脂肪变和炎细胞浸润等;血瘀型NAFLD大鼠血瘀体征积分及全血黏度、全血还原黏度及红细胞聚集指数较NAFLD组明显增高。结论:高脂饮食、NE及BSA复合因素可成功建立血瘀型NAFLD模型,该模型既具备非酒精性脂肪性肝病的病理特征,又符合中医血瘀证的证候表现。

Establishment and evaluation of combining disease and syndrome model of non-alcoholic fatty liver disease with syndrome of blood stasis

Objective: To establish a model combining disease and syndrome of non-alcoholic fatty liver disease with syndrome of blood stasis.Methods: 76 rats were randomly divided into normal group of 10,NAFLD group of 30 and blood stasis NAFLD group of 36.The rats in normal group were fed with normal diet,other groups were fed with high-fat diet.Simultaneously,the rats in the blood stasis NAFLD group were dealt with norepinephrine(NE) and bull serum albumin(BSA).At the end of 4th,8th and 12th weeks,the rats were disposed.Physical signs were observed,skin micro-circulation was detected with DRT4 laser Doppler blood stream tester.Hemarheology was tested with automatic blood rheology detector,serum lipidsa and serum ALT and AST were examined with automatic biochemistry analyser,fatty degeneration and inflammation of liver were observed with oil red O and HE dyeing.Results: The rats of blood stasis NAFLD group showed sublingual varicose veins,cyanoderma tongue with ecchymosis,black red eye ball,tail ecchymosis,skin micro-circulatory disturbance,abnormity of hemarheology,disorder of serum lipids and fatty degeneration and inflammatory cell infiltration.The integration of blood stasis physical signs and whole blood viscosity,whole blood reduction viscosity and RBC aggregation index in the blood stasis NAFLD group were higher than those in the NAFLD group.Conclusion: Blood stasis NAFLD model can be successfully induced by high-fat diet,NE and BAS.This model has either the characteristic of pathology of NAFLD,or the syndrome performance of blood stasis of TCM.