| 绿色荧光蛋白转染标记腺样囊性癌生长的裸鼠模型 |
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| 引用本文： | Chen ZG,Dong Z,Zhang D,Zhou C,Sun S,Liu S,Wei F. 绿色荧光蛋白转染标记腺样囊性癌生长的裸鼠模型[J]. 华西口腔医学杂志, 2011, 29(4): 424-428. DOI: 10.3969/j.issn.1000-1182.2011.04.023 |
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| 作者姓名： | Chen ZG Dong Z Zhang D Zhou C Sun S Liu S Wei F |
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| 作者单位： | 1.山东大学齐鲁医院口腔颌面外科, 山东大学口腔医学研究所, 济南250012;2.山东大学第二医院病理科, 济南250033; 3.山东大学口腔医院病理科, 济南250012 |
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| 基金项目： | 国家自然科学基金资助项目(30672339); 教育部留学回国人员科研启动基金资助项目(2007) |
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| 摘 要： | 目的 建立能够实时监测、连续动态观察腺样囊性癌生长、转移的裸鼠模型.方法 用逆转录病毒载体pLEGFP-N1感染腺样囊性癌细胞系ACCM并筛选出ACCM-GFP稳定细胞株,并比较ACCM和ACCM-GFP细胞的增殖特性和形态特点;用ACCM-GFP细胞种植法建立裸鼠舌癌模型,对移植瘤的生长进行实时荧光成像观察,并与AC...
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| 关 键 词： | 腺样囊性癌 绿色荧光蛋白 肿瘤模型 动物模型 |
| 收稿时间： | 2011-08-25 |
| 修稿时间： | 2011-08-25 |
Establishment of a green fluorescent protein-labeled mouse model of adenoid cystic carcinoma |
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| Chen Zheng-gang,Dong Zuoqing,Zhang Dong,Zhou Chengjun,Sun Shanzhen,Liu Shaohua,Wei Fengcai. Establishment of a green fluorescent protein-labeled mouse model of adenoid cystic carcinoma[J]. West China journal of stomatology, 2011, 29(4): 424-428. DOI: 10.3969/j.issn.1000-1182.2011.04.023 |
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| Authors: | Chen Zheng-gang Dong Zuoqing Zhang Dong Zhou Chengjun Sun Shanzhen Liu Shaohua Wei Fengcai |
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| Affiliation: | 1. Dept. of Oral and Maxillofacial Surgery, Qilu Hospital, Shandong University; Stomatology Center of Shandong University, Jinan 250012, China; 2. Dept. of Pathology, The Second Hospital of Shandong University, Jinan 250033, China; 3. Dept. of Pathology, School of Stomatology, Shandong University, Jinan 250012, China |
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| Abstract: | Objective To establish a novel nude mice model which can be visualized in real time and detected in a continuous and dynamic way for the development and metastasis of adenoid cystic carcinoma.Methods Human adenoid cystic carcinoma cells,ACCM cell line,were infected with retroviral vector of pLEGFP-N1 and then screened for a single colony of ACCM-GFP cells.Cell proliferation and morphological analysis were conducted for ACCM and ACCM-GFP cells.Nude mice lingual carcinoma model was set up with ACCM-GFP cells ... |
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| Keywords: | adenoid cystic carcinoma green fluorescent protein tumor model animal model |
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