树突状细胞免疫功能与慢性丙型肝炎病毒感染相关分析

目的:探讨树突状细胞(DC)与慢性丙型肝炎患者体内病毒持续感染的关系。方法:密度梯度离心法分离外周血单个核细胞(PBMC),贴壁培养2 h,吸去悬浮细胞,联合应用GM-CSF、IL-4刺激正常人及HC患者外周血DCD的增殖及成熟。观察DC外部形态;流式细胞仪检测各组的DC数量(CMRF-44标记法)及DC表面CD86、CD80、HLA-DR的表达水平;ELISA法检测培养7 d的DC上清中IL-12和IFN-的水平。结果:实验组DC细胞数为(7.5±3.3)×106/L,明显低于对照组的(14.9±5.1)×106/L(P<0.01)。实验组DC表面CD86、CD80和HLA-DR表达的阳性率分别为(52.16±13.12)%(、37.26±11.31)%、(29.76±13.34)%,对照组分别为(83.21±15.22)%(、47.93±12.19)%(、38.26±10.58)%,两者相差明显(P<0.05)。培养上清中IL-12平均值为(398±105)ng/L,干扰素γ平均值为(7912±832)ng/L,分别低于对照组(813±216)ng/L和(13407±1532)ng/L(P<0.05)。结论:DC的免疫功能低下与慢性丙型肝炎患者HCV感染的持续化有关。

Correlative Analysis of Dendritic Cell Function and Chronic Hepatitis C Virus Infection

Objective:To explore the relationship between dendritic cell(DC) and the persistence of HCV in chronic hepatitis C patients.Methods:The density centrifugation was employed to separate mononuclear cells from the periphral blood.After culture for 2 hours in tissue culture flask,the suspended cells were removed and GM-CSF,IL-4 were used to stimulate the proliferation and maturation of the DCs from normal persons and chronic hepatitis C patients.The shape of DCs was observed,and the mount of DCs and the expression levels of HLA-DR and CD80、CD86 on DC were detected by CMRF-44 mAb labelling and flow cytometer;The levels of IL-12 and IFN-γ in supernatants were measured by ELISA at 7 days after culture.Results:The DC counts in trauma groups(7.48±3.2)×106/L were obviously lower than in control groups(15.1±4.98)×106/L(P<0.05).The expression rate of CD86,CD80 and HLA-DR on HCV-DC were(52.16±13.12)%,(37.26±11.31)% and(29.76±13.34)% respectively,whereas those on normal-DC were(83.21±15.22)%,(47.93±12.19)%,and(38.26±10.58)%,respectively(P< 0.05).The average level of IL-12 and interferon(IFN) γ in HCV-DC was(398±105) ng/L and(7912±832) ng/L,which was obviously lower than(813±216) ng/L and(13407±1532) ng/L(P<0.05) in the normal-DC respectively(P<0.05).Conclusion:The function of DC in patients with chronic hepatitis C is decreased,which suggest that it is related to the persistent infection of HCV.