HPLC法测定护肝宁片中丹参酮Ⅱ_A的含量

目的:建立以高效液相色谱法测定护肝宁片中丹参酮ⅡA含量的方法。方法:色谱柱为Kromasil C18(200mm×4.6mm,5μm),流动相为甲醇-水(80∶20),柱温为25℃,流速为1.0mL·min-1,检测波长为270nm。结果:丹参酮ⅡA检测浓度在4.999～44.999μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.999 7);平均回收率为99.07%,RSD=0.33%(n=9)。结论:本方法灵敏、准确、重现性好,可用于护肝宁片的质量控制。

Determination of Tanshinone Ⅱ A in Huganning Tablets by HPLC

OBJECTIVE： To establish an HPLC method for the determination of Tanshinone ⅡA in Huganning tablets. METHODS： The determination was performed by HPLC using C18 column（200 mm × 4.6 mm, 5 μm） with the column temperature maintained at 25℃ . The mobile phase consisted of methanol- water （80 ： 20） at a flow rate of 1.0 mL · min^ -1 and a UV detection wavelength of 270 nm.RESULTS： The calibration curve of Tanshinone ⅡA was in good linearity over the range of 4.999-44.999μg · mL^-1（r = 0.999 7） and the mean recovery was 99.07% （RSD = 0.33%, n = 9） .CONCLUSION： The method is sensitive, accurate and reproducible, and it can be used for the quality control of Huganning tablets.