诱导骨髓基质细胞成血管平滑肌细胞的研究

目的 探讨体外诱导犬骨髓基质细胞(SMSC)为血管平滑肌细胞(VSMC)及其成血管的能力.方法 用含血小板源性生长因子(PDGF-BB)10ìg/L的内皮细胞培养液-2(EGM-2)诱导,无PDGF-BB的EGM-2培养液为对照,检测a-平滑肌肌动蛋白(a-SM actin)与肌钙结合蛋白(calponin)的表达并接种于聚羟基乙酸(PGA)培养4周后取材.结果 诱导组(n=5)细胞逐步呈平滑肌样转变,表达a-SM actin与calponin,流式细胞仪阳性率分别为(45.16±0.97)%、(37.54 4±1.15)%,可成血管样结构,对照组(n=5)变化不明显,阳性率(7.92±1.04)%、(6.37±0.83)%,成血管样结构能力差.结论 体外可诱导犬BMSC为VSMC并有成血管样结构的能力.

Induction of canine bone marrow stromal cells to vascular smooth muscle cells in vitro

Objective To investigate the feasibility of inducing canine BMSCs to VSMCs and the ability to form blood vessels. Methods BMSCs were cultured in EGM-2 plus I0 μg/L PDGF-BB as the experimental group and EGM-2 without PDGF-BB as the control one with α-SM actin and calponin tested. Then they were planted onto the PGA and cultured for 4 weeks before harvested. Results Induced BMSCs ( n = 5 ) showed morphological change to VSMCs, the expression of α-SM actin and calponin with the pesifive rate of (45.16 ±0.97)% and (37.54 ± 1.15) % respectively in FCM and the ability to form blood vessels. The control groups ( n = 5 ) had little change in morphology with positive rate ofα-SM actin and calponin being (7.92 ± 1.04) % and (6.37 ±0.83 ) % respectively in FCM and poor vessel forming ability. Conclusion BMSCs can be induced to VSMCs in vitro and also have the ability to form the blood vessel-like tissues.