依达拉奉对大鼠脑缺血急性期内质网应激的保护作用

目的：研究依达拉奉对大鼠脑组织缺血急性期引起的内质网应激（endoplasmic reticulum stress，ERS）的保护作用。方法：采用线栓法制备大鼠右大脑中动脉供血区缺血模型，通过脑组织含水量的测定和神经功能缺损评分来评价不同剂量（3．0、10．0mg／kg）依达拉奉的脑保护作用，并通过进一步检测与ERS相关的分子葡萄糖调节蛋白-78（GRP78）和caspase-12的表达量变化，来观察依达拉奉的脑保护作用与脑组织ERS的相关性。结果：不同剂量依达拉奉均可以使脑缺血急性期水肿的脑组织含水量减少，使大鼠神经功能评分改善，使大鼠脑缺血后高表达的GRP78出现下调，caspase-12的激活也受到抑制。结论：依达拉奉具有一定的脑保护作用，其机制可能与抑制脑组织缺血时的ERS有关。

Protective effects of edaravone against ischemia-induced endoplasmic reticulum stress in rats

Objective:To investigate the protective effects of edaravone against endoplasmic reticulum stress induced by brain ischemia.Methods:Focal cerebral ischemia model was made by Longa method in Sprague-Dawley rats.Water content of rat brain tissue was determined and neurological deficits after ischemia were assessed and scored.Western blot was used to determine the expression of GRP78 and activity of caspase-12.Results:The expression of GRP78 was elevated at 2,6,12,24 and 48 h after ischemia,and restored at 72 h;furthermore,caspase-12 was activated at 24 and 48 h after ischemia.Edaravone could significantly down-regulate the high expression of GRP78 and repress the activation of caspase-12.Conclusion:Edaravone has protective effects against brain ischemia and the mechanism of protective effect is possibly related to inhibition of endoplasmic reticulum stress.