Inflammation amplifies the antitumor cytostasis by human peritoneal macrophages |
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Authors: | Shlomo Ben-Efraim Corne Tak Marien J W A Fieren Gert-Jan C M van den Bemd Ivan L Bonta |
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Institution: | 1. Departments of Pharmacology, Erasmus University, 3000 DR, P.O. Box 1738, Rotterdam, The Netherlands 3. Internal Medicine, Erasmus University, 3000 DR, P.O. Box 1738, Rotterdam, The Netherlands
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Abstract: | The effect of an inflammatory environment on the antitumor cytostatic ability of human macrophages was examined. Peritoneal
macrophages of patients on continuous ambulatory peritoneal dialysis (CAPD) were collected, when CAPD was without complication,
during an intercurrent infectious inflammation and after recovery. Inhibition of3H-thymicline uptake served as a measure of cytostasis by macrophages co-cultured with target murine cells MOPC-315 plasmacytoma,
WEHI-3B rnyelomonocytic leukemia and L929 transformed fibroblasts. Macrophages from inflammatory peritoneum expressed a markedly
enhanced cytostasis, irrespective of the nature of the tumor cell. Endotoxin (LPS) challenge of inflammatory macrophages failed
to further reinforce the cytostasis towards MOPC-315 plasmacytoma, but reinforced the cytostasis towards WEHI-3B leukemia
(sensitive to inhibition by IL-1) and towards L929 (sensitive to TNFα). Cytostasis by supernatants of human peritoneal macrophages
against L929 was markedly inhibited by anti-rHuTNFa and against WEHI-3B by anti-rHuIL-lβ. The results suggest a link between
inflammatory function and antitumor cytostasis by macrophages. This link is constituted by mediators involved in the activation
process of macrophages. |
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Keywords: | Inflammation Antitumor activity Macrophages |
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