精液白细胞与精子形态学参数的关系

目的：探讨精液白细胞与精子形态学参数之间关系。 方法：对278例不育患者采用精子形态检测系统下人工修正方法进行精子形态分析，采用精液白细胞计数法检测精液白细胞，采用改良巴氏染色法分析精子顶体形态。结果：白细胞精子症组形态正常精子率和顶体完整率均低于非白细胞精子症组（P<0.05）；形态正常精子率与精液白细胞浓度呈负相关关系（r=-0.243，P<0.01）；顶体完整率与精液白细胞浓度亦呈显著负相关关系（ r=-0.248，P<0.01）。结论：精液白细胞增多可能影响精子的形态和顶体的完整。

Relationship between leukocyte concentration in semen and sperm morphological parameters

Objective To investigate the relationship between WBC concentration in semen and sperm morphological parameters. Methods Semen samples were collected from 278 infertile men. WBC in semen was detected through WBC count by microscope. Sperm morphology was evaluated using artificially modified method by Automated Sperm Morphology Analyzer. Acrosomal morphology was analysed with modified Papanicolaou staining. Results The percentage of morphologically normal sperm and the rate of acrosomal intactness in leukocytospermic group were both significantly lower than those in nonleukocytospermic group (P<0. 05). The WBC concentration in semen had significantly negative correlation with the percentage of morphologically normal sperm and the rate of acrosomal intactness (r= - 0.243, P<0.01; r=- 0.248, P<0.01). Conclusion Excessively high WBC concentration in semen could affect morphology of sperm and acrosomal intactness.