Acrylamide-induced astrogliotic and apoptotic responses in human astrocytoma cells |
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Authors: | Jong-Hang Chen Kuen-Yuh Wu Ing-Ming Chiu Tsui-Chun Tsou Chin-Cheng Chou |
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Institution: | 1. Department of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 106, Taiwan, ROC;2. Institute of Occupational Medicine and Industrial Hygiene, National Taiwan University, No. 17, Shiujou Road, Taipei 106, Taiwan, ROC;3. Institute of Cellular and System Medicine, National Health Research Institutes, No. 35, Keyan Road, Miaoli 350, Taiwan, ROC;4. Department of Internal Medicine, The Ohio State University, 480 West Ninth Avenue, Columbus, OH 43210, USA;5. Division of Environmental Health and Occupational Medicine, National Health Research Institutes, No. 35, Keyan Road, Miaoli 350, Taiwan, ROC;6. Center for Zoonoses Research, College of Bio-Resources and Agriculture, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 106, Taiwan, ROC |
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Abstract: | This study was to clarify whether acrylamide (ACR) will induce apoptosis and astrogliosis in an astrocytic cell line in vitro. Different time- and dose-dependent cytotoxic studies were conducted upon neuronal (SH-SY5Y) and glial cell lines (U-1240 MG) under exposure to ACR up to 72 h. We showed that SH-SY5Y cells were more sensitive in cytotoxic assays than U-1240 MG cells, and significantly decreased cell viability was observed at concentrations higher than 1 mM with increased lactate dehydrogenase leakage observed only at 5 and 10 mM in U-1240 MG cells. The ACR-induced apoptotic responses and phosphorylation of p53 protein at Ser15 for U-1240 MG cells were identified at 48 h. The increase of glial fibrillary acidic protein (GFAP) as a chemical-induced astrogliotic response was found to be associated with different ACR concentrations and exposure times, particularly at ≧48 h of ≧2 mM. In addition, immunocytochemical staining at 36 h of 5 and 10 mM treatments had significantly higher density of GFAP than the control. Thus, ACR-induced effects can be seen in neuronal and astrocytic cells. These results suggest that ACR exposure may lead to apoptotic and astrogliotic effects in human astrocytoma cells in vitro in a time- and dose-dependent manner. |
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