声学微泡造影技术测定动物模型中肺血量的可行性及可重复性研究

  目的  通过动物实验论证超声微泡造影技术测量肺血量（pulmonary blood volume, PBV）的可行性及可重复性。  方法  采用“声诺维”造影剂，利用超声微泡造影技术测量动物模型肺通过时间（pulmonary transit time, PTT）或标准化的肺通过时间（heart rate-normalized pulmonary transit time, nPTT）计算PBV，根据观察者组内、组间变异性，个体观察者日内变异性以探讨其可重复性，通过在急性缺血性左心衰动物模型中的应用以评估其可行性。  结果  Bland-Atlman法统计结果显示该测量方法测量结果观察者组内一致性、日内一致性和组间一致性均较好；在急性缺血性左心衰动物模型中的应用观察发现:PTT〔（2.67±0.28） s〕较心衰前PTT〔（1.75±0.07） s〕延长，nPTT（6.72±0.57）较心衰前nPTT（3.93±0.16）增加，PBV〔（168.52±21.24） mL〕较心衰前PBV〔（108.79±11.98） mL〕增多，差异均有统计学意义（P<0.05）。  结论  超声微泡造影技术检测PTT或nPTT计算PBV简易可行，不易产生误判，不同观察者间检测结果一致，重复性检测结果一致性较高；通过该方法测量PTT或nPTT计算并比较PBV的变化具有一定的可行性。

The Feasibility and Repeatability of Ultrasound Microbubble Angiography in the Determination of Pulmonary Blood Volume in Animal Models

  Objective  To investigate the feasibility of measuring pulmonary blood volume (PBV) by ultrasound microbubble angiography, which may provide a feasible method for further detection of PBV changes.  Methods  Ultrasound microbubble angiography was used to calculate the PBV by detecting pulmonary transit time (PTT) and heart rate-normalized pulmonary transit time (nPTT). To evaluate the consensus degree based on the intra-, inter-observer and within-day variation in order to determine the repeatability. The method was used for acute left ventricular dysfunction models to determine the feasibility as well.  Results  The Bland-Atlman plots showed good intra-observer, within-day, and inter-observer consistency of measurement results. Application in acute left ventricular dysfunction models showed that, compared with the control, left heart failure models had higher PTT, nPTT and PBV (P<0.05).  Conclusion  Detection of PTT/nPTT to calculate PBV by ultrasound microbubble angiography is simple and feasible, it is not easy to produce miscarriage of justice, with good intra- and inter-observer consistency of repeatability test results. The method has certain feasibility.