人参皂苷Rg1联合抗生素治疗小鼠脓毒症急性肺损伤

  目的  观察人参皂苷Rg1联合抗生素亚胺培南对脓毒症急性肺损伤的影响。  方法  C57BL/6雄性小鼠以盲肠穿刺法建立脓毒症模型，将模型小鼠随机分为模型组、亚胺培南组、人参皂苷Rg1组、人参皂苷Rg1+亚胺培南组，每组10只。另取10只C57BL/6小鼠只开腹不穿刺，作为假手术组。各组于术后2 h、26 h、50 h腹腔注射给药。末次给药2 h后（术后52 h），取小鼠动脉血液测定氧合指数，取肺组织测定肺脏湿/干质量比，HE染色观察肺脏炎症，ELISA测试盒检测肺泡灌洗液白介素（IL）-1β、IL-6、肿瘤坏死因子（TNF）-α及核转录因子（NF）-κB的水平，蛋白印迹及免疫组织化学染色检测肺组织NF-κB p65的表达。  结果  各给药组均能够显著降低脓毒症小鼠的肺脏湿/干质量比，并升高血液中的氧合指数（P<0.01），降低肺泡灌洗液中IL-1β、IL-6、TNF-α及NF-κB水平（P<0.01），同时降低肺组织中NF-κB p65的表达（P<0.01）。人参皂苷Rg1合并亚胺培南应用时，与单用人参皂苷Rg1及单用亚胺培南组相比，上述指标更接近对照组水平。HE染色结果显示给药治疗后的各组脓毒症小鼠的肺泡炎细胞浸润减轻，联合用药组的肺脏组织形态与假手术组最为接近。  结论  人参皂苷Rg1单用和联用均能够抑制脓毒症小鼠的肺脏炎症，以抗生素治疗脓毒症时或可考虑联合使用。

Experimental Study of Ginsenoside Rg1 Combined with Antibiotics in the Treatment of Acute Lung Injury in Mice with Sepsis

  Objective  To observe the effect of ginsenoside Rg1 on the acute lung injury of sepsis in combination with the antibiotic imipenem in a mouse model of sepsis that induced by cecal puncture.  Methods  C57BL/6 mice were used to establish the model of sepsis. The model mice were randomly divided into model group, imipenem group, ginsenoside Rg1 group, and ginsenoside Rg1+imipenem group, 10 mice in each group. Another 10 mice were set as control group. Sham operation was performed in the mice of control group. Each mice was intraperitoneally injected the corresponding drug in 2 h, 26 h and 50 h after surgery for three times. They were 2 h after surgery, 26 h after surgery and 50 h after surgery. 2 h after the last administration, the oxygenation index of the arterial blood was measured, the lung tissue was taken to measure lung wet/dry ratio (W/D), and HE staining was used to observe the lung inflammation. The ELISA kits were used to detect the levels of interleukin (IL)- 1β, IL-6, tumor necrosis factor (TNF)-α and nuclear factor-kappa B (NF-κB) inalveolar lavage fluid. Western blot and immunohistochemical staining were used to detect NF-κB p65 expression in lung tissues.  Results  The drug-administered groups significantly reduced the W/D of the lungs in the septic mice and increased the oxygenation index in the blood (P<0.01), and decreased the inflammation in lung and the levels of IL-1β, IL-6, TNF-α and NF-κB in the alveolar lavage fluid (P<0.01), and decreased the expression of NF-κB p65 in lung tissue (P<0.01). When ginsenoside Rg1 was combined with imipenem, the above indicators were closer to the control group than that in the ginsenoside Rg1 and imipenem groups.  Conclusion  Rg1 can significantly inhibit lung inflammation in septic mice. It has a better therapeutic effect when combined with antibiotics.