UHRF1调控雌激素受体表达对甲状腺乳头状癌细胞增殖、侵袭和迁移的影响

  目的  观察泛素样含PDH和环指域1 (UHRF1)对雌激素受体（ER）α和ERβ表达比率的影响，探讨UHRF1影响甲状腺乳头状癌BCPAP细胞增殖、侵袭和迁移的机制。  方法  应用Western blot、实时荧光定量（qRT）-PCR检测正常甲状腺细胞Nthy-ori3-1和甲状腺乳头状癌细胞BCPAP中UHRF1、ERα和ERβ的蛋白及mRNA表达。分别用Scrambled siRNA、UHRF1 siRNA处理BCPAP细胞，qRT-PCR检测各组细胞中ERα和ERβ的mRNA表达；MTT、Transwell分别检测各组细胞增殖、侵袭和迁移。  结果  与Nthy-ori3-1细胞相比，BCPAP细胞中UHRF1和ERα蛋白及mRNA表达水平上调（P<0.05），而ERβ蛋白和mRNA表达水平下调（P<0.05）。与空白对照组和Scrambled siRNA组相比，转染UHRF1 siRNA的BCPAP细胞中ERα mRNA表达降低（P<0.05），ERβ mRNA表达升高（P<0.05）；转染UHRF1 siRNA的BCPAP细胞增殖、侵袭和迁移降低（P<0.05）。  结论  UHRF1可上调ERα/ERβ的表达比率，促进甲状腺乳头状癌BCPAP细胞增殖、侵袭和迁移。

Effects of UHRF1 on Estrogen Receptor and Proliferation,Invasion and Migration of BCPAP Cells in Thyroid Papillary Carcinoma

  Objective  To investigate the effects of ubiquitin-like PDH and ring finger domain 1 (UHRF1) on the expression ratio of estrogen receptor (ER) α/ERβ, and to explore the experimental mechanism of UHRF1 affecting the proliferation, invasion and migration of BCPAP cells in papillary thyroid carcinoma.  Methods  The protein and mRNA expressions of UHRF1, ERα and ERβ in normal thyroid Nthy-ori3-1 cells and thyroid papillary carcinoma BCPAP cells were detected by Western blot and qRT-PCR. BCPAP cells were treated with Scrambled siRNA and UHRF1 siRNA, respectively. The expressions of ERα and ERβ mRNAs were detected by qRT-PCR. MTT and Transwell were used to determine the proliferation, invasion and migration in each group of BCPAP cells.  Results  Compared with Nthy-ori3-1 cells, the expressions of UHRF1 and ERα proteins and mRNAs in BCPAP cells were significantly up-regulated (P<0.05), while the expressions of ERβ protein and mRNA were significantly down-regulated (P<0.05). Compared with the control group and Scrambled siRNA group, the expression of ERα mRNA in BCPAP cells transfected with UHRF1 siRNA was significantly decreased (P<0.05), while the expression of ERβ mRNA was significantly increased (P<0.05). The proliferation, invasion and migration of BCPAP cells transfected with UHRF1 siRNA were significantly decreased (P<0.05).  Conclusion  UHRF1 upregulates ERα/ERβ expression ratio and promotes proliferation, invasion and migration of BCPAP cells in papillary thyroid carcinoma.