七氟醚预处理通过升高血浆脂联素水平保护心肌缺血/再灌注损伤

目的探讨脂联素是否参与七氟醚预处理对心肌缺血/再灌注(myocardial ischemia-reperfusion,MI/R)损伤的保护作用。方法将C57小鼠随机分为4组,每组10只:Sham-Control(假手术组)、MI/R-control(模型组)、MI/R-Sevopre(预处理组)、APN-KO-MI/R-Sevopre(敲基因预处理组),MI/R造模术后24 h检测各组小鼠血浆脂联素水平;检测各组小鼠24 h左心室收缩末期内径,舒张末期内径以及射血分数;Tunel染色观察各组心肌细胞形态和凋亡情况;伊文思蓝-TTC染色观察心梗面积。结果相比于假手术组,模型组心功能受损,射血分数降低,舒张末期内径以及收缩末期内径增加,同时,模型组心肌梗死面积显著增加,凋亡细胞增多,并且血浆脂连素水平降低;相比于模型组,七氟醚预处理组小鼠心功能改善,心肌梗死面积降低,凋亡细胞减少,血浆脂连素水平升高;敲基因预处理组小鼠相比于预处理组,七氟醚预处理改善MI/R损伤作用降低。结论七氟醚预处理通过升高血浆脂连素水平保护MI/R损伤。

Sevoflurane preconditioning protects myocardial ischemia/reperfusion injury by increasing plasma adiponectin levels

Aim To investigate whether adiponectin is involved in the protective effect of sevoflurane preconditioning on myocardial ischemia/reperfusion(MI/R)injury.Methods C57 mice were randomly divided into four groups:the Sham Control(sham group),the MI/R-Control(model group),the MI/R-Sevopre(pretreatment group)and the APN-KO-MI/R-Sevopre(knockout pretreatment group).The plasma adiponectin level was detected 24 h after MI/R modeling.Meanwhile,the left ventricular end-systolic diameter,end-diastolic diameter and ejection fraction were measured at 24 h.In addition,TUNEL staining was used to detect the left ventricular end-systolic diameter,end-diastolic diameter and ejection fraction and the morphology and apoptosis of myocardial cells in each group were observed.What’s more,the infarct area was observed by Evans blue TTC staining.Results Compared with sham group,model group had impaired heart function,decreased ejection fraction and increased end-diastolic and end-systolic diameters.Meanwhile,the myocardial infarction area and apoptotic cells significantly increased in model group,and the plasma adiponectin level decreased.However,compared with model group,mice in sevoflurane pretreatment group had improved heart function,decreased myocardial infarction area,decreased apoptotic cells and increased plasma adiponectin levels.And then,compared with pretreatment group,sevoflurane pretreatment in knockout pretreatment group reduced MI/R injury.Conclusion The sevoflurane pretreatment protects MI/R injury by increasing plasma adiponectin levels.