| 血浆miR-1228-5p、miR-34a-5p、miR-192-5p和miR-30a-3p水平与早发冠心病的相关性及其初筛价值 |
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| 引用本文： | 田开疆,周坤,桂丹丹,罗雯,欧阳劭,马小峰,刘欢,胡恒境,姜志胜. 血浆miR-1228-5p、miR-34a-5p、miR-192-5p和miR-30a-3p水平与早发冠心病的相关性及其初筛价值[J]. 中国动脉硬化杂志, 2022, 30(8): 681-690 |
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| 作者姓名： | 田开疆 周坤 桂丹丹 罗雯 欧阳劭 马小峰 刘欢 胡恒境 姜志胜 |
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| 作者单位： | 南华大学心血管疾病研究所 动脉硬化学湖南省重点实验室 湖南省动脉硬化性疾病国际科技创新合作基地,;南华大学附属第二医院心血管内科,湖南省衡阳市 421001;南华大学附属南华医院心血管内科, 湖南省衡阳市 421002;南华大学附属第一医院心血管内科,湖南省衡阳市 421001 |
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| 基金项目： | 国家自然科学基金项目(91839103、81670429和81470435)；湖南省重点研发计划(2020SK2105) |
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| 摘 要： | 目的]探讨血浆miR-1228-5p、miR-34a-5p、miR-192-5p和miR-30a-3p水平与早发冠心病(PCAD)的相关性及其对PCAD的初筛价值。[方法]根据纳入标准及排除标准,纳入6例明确诊断的PCAD患者作为PCAD组,纳入6例健康受试者作为对照组,收集PCAD组和对照组血液,提取血清样本并保存,使用DNBseq平台检测两组血清中miRNA水平,筛选差异水平显著的miRNA。根据纳入标准及排除标准,收集78例PCAD患者、75例晚发冠心病患者和69例健康对照者的血液并对筛选的miRNA进行实时荧光定量PCR验证。分析PCAD患者冠状动脉造影报告,采用Gensini评分评估冠状动脉病变的严重程度。Spearman相关性检验分析有关miRNA水平与冠状动脉狭窄程度的相关性。ROC曲线分析血浆miR-1228-5p、miR-34a-5p、miR-192-5p及miR-30a-3p水平对PCAD的诊断价值,多因素Logistic回归分析PCAD发生的影响因素。[结果]DNBseq平台分析显示,差异表达miRNA 33个,其中上调miRNA 17个,下调miRNA 16个,差异水平最为显著的5个miRNA分别为miR-1228-5p、miR-34a-5p、miR-192-5p、miR-424-3p和miR-30a-3p；实时荧光定量PCR结果显示,与对照组相比,PCAD患者血浆miR-1228-5p升高1.7倍,miR-34a-5p升高1.4倍,miR-192-5p升高0.7倍,miR-30a-3p升高2.5倍(P＜0.05),两组间血浆miR-424-3p水平差异无统计学意义(P＞0.05)；血浆miR-1228-5p和miR-34a-5p水平与PCAD患者冠状动脉狭窄程度均呈正相关(r＝0.307,P＝0.004；r＝0.238,P＝0.036)；ROC曲线分析显示,miR-1228-5p、miR-34a-5p、miR-192-5p和miR-30a-3p诊断PCAD的ROC曲线下面积分别为0.903、0.832、0.731及0.798,其联合诊断PCAD的ROC曲线下面积为0.990,95%CI为0.976～1.000。[结论]PCAD患者血浆miR-1228-5p、miR-34a-5p、miR-192-5p和miR-30a-3p水平显著升高,其联合检测诊断PCAD具有较高的准确性,有望成为初筛PCAD的新型生物标志物。
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| 关 键 词： | 早发冠心病 新型生物标志物 miR-1228-5p miR-34a-5p miR-192-5p miR-30a-3p |
| 收稿时间： | 2022-03-03 |
| 修稿时间： | 2022-05-06 |
Correlation between plasma miR-1228-5p, miR-34a-5p, miR-192-5p and miR-30a-3p levels and premature coronary artery disease and its preliminary screening value |
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| TIAN Kaijiang,ZHOU Kun,GUI Dandan,LUO Wen,OU-YANG Shao,MA Xiaofeng,LIU Huan,HU Hengjing,JIANG Zhisheng. Correlation between plasma miR-1228-5p, miR-34a-5p, miR-192-5p and miR-30a-3p levels and premature coronary artery disease and its preliminary screening value[J]. Chinese Journal of Arteriosclerosis, 2022, 30(8): 681-690 |
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| Authors: | TIAN Kaijiang ZHOU Kun GUI Dandan LUO Wen OU-YANG Shao MA Xiaofeng LIU Huan HU Hengjing JIANG Zhisheng |
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| Affiliation: | Institute of Cardiovascular Disease, University of South China & Key Laboratory for Arteriosclerology of Hunan Province & International Joint Laboratory for Arteriosclerotic Disease Research of Hunan Province, Hengyang, Hunan 421001, China;Department of Cardiology, the Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China;Department of Cardiology, the Affiliated Nanhua Hospital of University of South China, Hengyang, Hunan 421002, China;Department of Cardiology, the First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China |
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| Abstract: | |
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| Keywords: | premature coronary artery disease novel biomarker miR-1228-5p miR-34a-5p miR-192-5p miR-30a-3p |
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