鼻咽癌EMT相关基因的筛选及生物信息学分析

目的利用生物信息学方法挖掘鼻咽癌上皮间充质转化（epithelial-mesenchymal transition，EMT）发生的潜在分子机制。方法从公共基因芯片数据库GEO（gene expression omnibus）中寻找并下载鼻咽癌的相关基因芯片数据，并使用Genclip软件对下载的数据进行分析，寻找鼻咽癌公共基因芯片数据中与EMT相关的基因，并用生物信息学方法对筛选出来的基因作进一步分析。结果在公共的鼻咽癌芯片数据中找到35个与EMT相关的差异表达基因，这些基因功能大致与细胞组分、细胞粘附、通信、信号转导、分化、运动、迁移以及细胞表面受体相关的信号转导等有关，这些功能往往都被认为与肿瘤的侵袭和转移有关。结论利用生物信息学的方法能有效分析基因芯片数据并获取生物内在信息。鼻咽癌EMT是由于多种基因表达改变所致，这为确定鼻咽癌早期转移诊断标志与预后的预示开辟了新的思路。

Screening of Nasopharyngeal Carcinoma EMT-related Genes and Their Bioinformatics Analysis

Objective To identify the molecular mechanism of EMT in nasopharyngeal carcinoma by bioinformatics methods. Methods The gene chip data of nasopharyngeal carcinoma were obtained from GEO database and analyzed by GenClip software to identify the genes related to EMT in nasopharyngeal carcinoma with bioinformatics analysis. Results 35 differentially expressed EMT-related genes were identified in the public gene chip database of nasopharyngeal carcinoma.These genes were associated with the cellular components,adhesion,communication,signal transduction, differentiation, motion, migration and cell surface receptor-linked signal transduetion which attribute to the tumor invasion and metastasis. Conclusion Bioinformaties analysis can help with effective analysis of the gene chip data.The pathogenesis of EMT in nasopharyngeal carcinoma involves abnormal expression of multiple genes, and these data may benefit further investigations of the early diagnosis and treatment of the malignancy.