喉癌病人外周血淋巴细胞端粒酶活性的检测及临床意义

①目的　探讨外周血淋巴细胞端粒酶活性与喉癌的相关性。②方法　采用聚合酶链反应基础上端粒重复序列扩增法(PCR TRAP)检测喉癌病人、声带息肉病人及正常人外周血淋巴细胞中端粒酶活性。③结果喉癌病人43例,有37例端粒酶阳性,阳性率86.05%;21例声带息肉病人中有 3 例阳性,阳性率 14.29%;20 例正常人中有2例阳性,阳性率10.00%。端粒酶活性在喉癌病人与声带息肉病人、喉癌病人与正常人之间的差异有显著性(uc=3.65、5.45,P<0.01);声带息肉病人与正常人之间差异无显著性(uc=0.44,P>0.05)。④结论　端粒酶表达在喉癌的发病机制中可能起着重要作用,喉癌病人外周血淋巴细胞端粒酶活性的测定,对喉癌的早期诊断、治疗等都有重要的临床意义。

DETECTION OF TELOMERASE ACTIVITY IN LYMPHOCYTES OF PERIPHERAL BLOOD OF PATIENTS WITH LARYNGEAL CARCINOMAS AND ITS CLINICOPATHOLOGIC SIGNIFICANCE

Objective To investigate the correlation between telomerase activity in lymphocytes of peripheral blood and laryngeal carcinomas. Methods Telomerase activity in peripheral lymphocytes in patients with laryngeal carcinomas and laryngeal polyps and normal persons were determined by PCR-TRAP. Results Telomerase activity was detected in 37 of 43 laryngeal carcinomas (86.05%), three of 21 laryngeal polyps (14.29%) and two of 20 normal persons (10.00%). The telomerase activity was significantly different between laryngeal carcinomas and laryngeal polyps, and between laryngeal carcinomas and normal persons (u_c=3.65,5.45;P<0.01), but not between laryngeal polyps and normal persons (u_c=0.44,P>0.05). Conclusion Telomerase activity may play a crucial role in the tumorigenesis of laryngocarcinoma. Detection of telomerase activity in lymphocytes of peripheral blood in laryngeal carcinomas is of great importance to early diagnosis and treatment of laryngeal carcinomas.